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Optimizing methylation conditions for gas liquid chromatography assay of lactic acid and succinic acid in biological samples



Optimizing methylation conditions for gas liquid chromatography assay of lactic acid and succinic acid in biological samples



Chromatographia 12(1): 22-24



Aqueous standard solutions of lactic and succinic acids were methylated for assay by GLC. The efficiency of methylation using boron trifluoride-methanol reagent was compared to methylation using a sulfuric acid-methanol mixture; the ratio of sulfuric acid and methanol was also varied. Reactions were done at 100.degree. C for 5 min and at room temperature 20 h. The sulfuric acid-methanol method was most efficient for a rapid assay; however, the ratio of sulfuric acid and methanol used is critical in the assay of lactic acid and would be especially important in the clinical laboratory using GLC for analysis of CSF from suspected bacterial meningitis patients or other body fluids.

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Accession: 006039382

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