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Orientation of the hemes of high potential cytochromes relative to photosynthetic membranes as shown by the linear dichroism of oriented preparations



Orientation of the hemes of high potential cytochromes relative to photosynthetic membranes as shown by the linear dichroism of oriented preparations



Biochimica et Biophysica Acta 593(2): 299-311



The orientations of high potential cytochromes with respect to photosynthetic membranes were investigated in spinach chloroplasts and in Rhodopseudomonas viridis. The approach consisted in detection with polarized light of photoinduced absorbance changes related to the oxidation of the cytochromes. The orientation of cytochrome c-558 was measured at room temperature in chromatophores and whole cells of R. viridis, oriented on glass slides and in a magnetic field, respectively. The orientation of cytochrome b-559 of green plants was detected at 77.degree. K in magnetically oriented chloroplasts. In both cases the dichroic ratio for the .alpha. band showed that the heme plane made an angle greater than 35.degree. C with the membrane plane. The dichroic ratio was not constant throughout the .alpha. and .beta. bands, for both cytochrome c-558 and b-559. Linear dichroism spectra of oriented pure horse heart cytochrome c and cytochrome c2 of R. sphaeroides in stretched polyvinyl alcohol films showed that the variations of the dichroic ratio in the .alpha. and .beta. bands may be explained by the occurrence of x- and y-polarized transitions absorbing at slightly different wavelengths.

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Accession: 006044426

Download citation: RISBibTeXText

PMID: 7236637

DOI: 10.1016/0005-2728(80)90067-5


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