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Oriented structure of pectic poly saccharides in pea pisum sativum cultivar alaska epidermal cell walls



Oriented structure of pectic poly saccharides in pea pisum sativum cultivar alaska epidermal cell walls



Plant & Cell Physiology 21(6): 999-1006



Polarized IR absorption spectra of film specimens of the epidermal cell wall of the 3rd internode of pea stems were recorded before and after treatment with endo-polygalacturonase (endo-PG) and endo-pectin lyase (endo-PL). Pectic polysaccharides solubilized with endo-PG were essentially the same as those solubilized with endo-PL. The degree of esterification of the pectic polysaccharides was .apprx. 20% and their major sugar components were uronic acids (32.8%), arabinose (48.1%) and galactose (19.2%). The polarized IR spectra showed that pectic polysaccharides have an oriented structure in cell walls with their molecular chains oriented preferentially parallel to the direction of cell elongation.

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