+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn

+ Translate
+ Recently Requested

Origin and genetic analysis of plants regenerated in vitro from periclinal chimeras of lycopersicon peruvianum



Origin and genetic analysis of plants regenerated in vitro from periclinal chimeras of lycopersicon peruvianum



Zeitschrift fuer Pflanzenzuechtung 77(2): 112-120



Periclinal chimeras of the type 2n-4n-4n and 4n-2n-2n obtained from anther cultures of the diploid S-genotype S1S3 of L. peruvianum were used to understand the origin of plants regenerated in vitro from stem cultures. Internodes excised from stem segments below the floral branches were cultured on either gand 5 [sucrose 2%, thiamine 0.4 mg/l, 2 eatin 2.19 mg/l] or Nitsch's medium. Shoot regeneration was obtained on gand 5 without any intermediate callus formation; with Nitsch's medium neither callus formation nor shoot regeneration occurred even after prolonged culturing. The importance of culture conditions and composition of media are discussed. The cytological analysis on the plants regenerated in vitro shows that they originated from the inner histogenetic layers, L2 and/or L3. The genetic analysis of the same plants allowed the isolation of a diploid plant, derived from a 4n-2n-2n chimera, in which some new S-allele was induced.

(PDF emailed within 1 workday: $29.90)

Accession: 006044772

Download citation: RISBibTeXText


Related references

Plant regeneration from in vitro cultures of anthers and stem internodes in an interspecific hybrid lycopersicon esculentum x lycopersicon peruvianum and cyto genetic analysis of the regenerated plants. Plant Science Letters 20(2): 157-166, 1980

Genetic instability at the s locus of lycopersicon peruvianum plants regenerated from in vitro culture of anthers generation of new s specificities and s allele reversions. Heredity 49(3): 319-330, 1982

Periclinal chimeras composed of Lycopersicon peruvianum and L esculentum demonstrate that S-locus associated proteins and self-incompatibility can be uncoupled. Hortscience 30(2): 193, 1995

Plant regeneration from in vitro cultures of stem internodes in self-incompatible triploid Lycopersicon peruvianum Mill. and cytogenetic analysis of regenerated plants. Plant Science Letters 22(3): 197-204, 1981

Chimerism in lycopersicon peruvianum plants regenerated from in vitro cultures of anthers and stem internodes. Zeitschrift fuer Pflanzenzuechtung 76(4): 299-319, 1976

Genetic analysis of the origin of plants regenerated from anther tissues of Lycopersicon esculentum Mill. Plant Science Letters 21(3): 223-227, 1981

Ontogenetic origin of in vitro - regenerated plants in Lycopersicum peruvianum. Incompatibility Newsletter (6): 76-79, 1975

Transgenic tobacco plants regenerated from leaf disks can be periclinal chimeras. Plant molecular biology: an international journal on molecular biology biochemistry and genetic engineering 21(4): 705-708, 1993

Genetic analyses of plants regenerated from in vitro culture of anthers and stem-internodes in Lycopersicum peruvianum. Incompatibility Newsletter (6): 33-34, 1975

Utilization of the Lycopersicon peruvianum Mill, x L. esculentum Mill, periclinal chimaera in the Lycopersicon genus genetics. Genet, polon, 10: 3-4, 70 p, 1969

Changing the genetic structure of tomato plants by the method of gamma fusion of lycopersicon esculentum mill. and lycopersicon peruvianum var dentatum dun. Biopolimery i Kletka 7(4): 82-91, 1991

Genetic variability in tomato (Lycopersicon esculentum) plants regenerated from in vitro cultured cotyledons. Genetica Agraria 38(3): 325, 1984

In vitro culture of anthers and stem internodes of Lycopersicum peruvianum: nuclear DNA determination in calli and cytological analysis of regenerated plants. Zeitschrift fur Pflanzenphysiologie 82(5): 377-388, 1977

Cross compatibility between the cultivated tomato lycopersicon esculentum and the wild species lycopersicon peruvianum lycopersicon chilense assessed by ovule culture in vitro. Japanese Journal of Breeding 41(2): 223-230, 1991

Genetic analysis of instability inPetunia hybrida : 3. Periclinal chimeras resulting from frequent mutations of unstable alleles. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 69(3): 297-304, 1985