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Osmotic shock fluids from salmonella r mutants chemical composition and protective capacity against salmonella typhimurium infection in mice



Osmotic shock fluids from salmonella r mutants chemical composition and protective capacity against salmonella typhimurium infection in mice



Zentralblatt fuer Bakteriologie Mikrobiologie und Hygiene 1 Abt Originale A 252(3): 347-354



Salmonella rough mutants of different chemotype were subjected to osmotic shock treatment. Chemical composition (protein, lipopolysaccharide, palmitic acid and nucleic acids) and polypeptide patterns of the shock fluid and hypertonic fluid (in which the cells were suspended before shock treatment) were determined and compared with material extracted by urea treatment carried out to obtain protein from the cell surface. Apparently, even components from the cell surface were released by shock treatment. All the extracts mentioned above showed protective activity against S. typhimurium infection in mice.

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