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Papain catalyzed synthesis of methionine enriched soy plasteins average chain length of the plastein peptides



Papain catalyzed synthesis of methionine enriched soy plasteins average chain length of the plastein peptides



Journal of Agricultural & Food Chemistry 27(6): 1281-1285



A plastein was synthesized from a peptic soy protein hydrolyzate and 14C-labeled methionine ethyl ester as substrates and with papain as a catalyst. The resulting water-insoluble plastein contained 14.3% of covalently linked methionine. By partial or complete oxidation of the plastein-linked methionine, it was converted into a water-soluble peptide mixture. This mixture was shown by gel chromatography on Sephadex G-25 to consist of peptide species of the same size as the substrate peptides used for plastein synthesis. Radioactive methionine was incorporated into the entire range of substrate peptides. The average chain length was established by chemical means to be about 6 residues for substrate and product peptides. No high-MW species were formed during plastein synthesis.

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Accession: 006059862

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