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Papain hydrolysis products in 4 mono clonal immuno globulin subclasses



Papain hydrolysis products in 4 mono clonal immuno globulin subclasses



Archivum Immunologiae et Therapiae Experimentalis 28(2): 265-274



Four different classes of monoclonal [human] IgG were submitted to papain digestion activated by cysteine. After digestion of subclasses G-1, 3 and 4 about 80% of the initial amount of IgG was converted to fragments Fab and Fc (less in subclass IgG2). In the initial time of digestion subfragments Fc', Facb-like and nonidentified product of digestion (fraction IV) are also obtained. Additional products (besides Fab and Fc) corresponded to 11-17% of the initial IgG amount. In contrast to fragments Fab and Fc the additional fragment amounts did not increase during the digestion time and the amount of Facb-like fragment in G-1 and G-3 subclasses even decreased at the end of hydrolysis. The Facb-like fragment may be an intermediate product of Ig digestion. IgG2 was resistant in 60% to 18 h of hydrolysis. Only 20% of IgG2 was digested to Fab and Fc fragments and 20% to other digestion products.

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