Partial separation and characterization of soluble phosphatases from leaves of wheat triticum aestivum cultivar gamenya grown under phosphorus deficiency and water deficit

Barrett Lennard, E.G.; Greenway, H.

Journal of Experimental Botany 35(135): 694-704


ISSN/ISBN: 0022-0957
Accession: 006066900

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Soluble phosphatases from wheat leaves were separated into 2 fractions by CM-cellulose chromatography. The phosphatase(s) in the fraction adsorbed by CM-cellulose (fraction B) were identical with an electrophoretic band of phosphatase activity which increased with P deficiency. The phosphatase activity in this fraction increased specifically as the concentration of Pi in the sampled tissues fell from 0.8% to 0.2% dry weight. Phosphatase activity in fraction B may be due to an isozyme of acid phosphatase. Phosphatase activity in the fraction not adsorbed by CM-cellulose (Fraction A) increased with P deficiency and water deficit and was higher in young than in mature leaves. The fraction A phosphatase which increased with water deficit was probably another isozyme of acid phosphatase; the fraction A phosphatase which increased with P deficiency was probably not an acid phosphatase because it had activity only against p-nitrophenylphosphate and ATP. In expanding leaves of P deficient plants, phosphatase activities in fraction A and B declined after P was supplied to adequate levels. In fully expanded leaves, high phosphatase activities persisted after the resupply of P. The use of measurements of phosphatase activity as a biochemical marker of P deficiency is discussed.