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Pathogenesis of 1 2 di methyl hydrazine induced carcinomas in rat intestine 2. histochemical and ultrastructural characteristics of glyco proteins of 1 2 di methyl hydrazine induced carcinoma cells



Pathogenesis of 1 2 di methyl hydrazine induced carcinomas in rat intestine 2. histochemical and ultrastructural characteristics of glyco proteins of 1 2 di methyl hydrazine induced carcinoma cells



Acta Pathologica Japonica 32(2): 265-280



Lesions (227) of DMH (1,2-dimethylhydrazine)-induced intestinal carcinomas of 68 male Wistar rats were investigated for histochemical and EM properties to study their histogenesis and mucinogenesis. Among the epithelial glycoproteins, surface glycoprotein on the microvilli with glycocalyces was distinguishable from mucin in the cytoplasm based on histochemical staining and ultrastructural findings. Based on the phenotypic characteristics of glycoproteins, DMH-induced carcinomas could be classified into mucin-producing carcinomas composed mainly of cells containing intracellular mucins and nonmucin producing cells. The mucin-producing carcinomas consisted of poorly differentiated adenocarcinoma, signet ring cell carcinoma and mucinous carcinoma, presumably resulting from various degrees of differentiation into goblet cells. Their staining attitude varied and cells with neutral mucin, sialomucin and sulfated mucin were observed in the signet ring cell carcinoma. Abnormal synthesis of glycoproteins was observed with Con[concanavalin]A-paradox staining and periodic acid-thionine Schiff/periodate-borohydride/saponification/periodic acid-Schiff. These histochemical properties suggested irregulatory synthesis of glycoproteins in carcinomas. In nonmucin-producing carcinomas, glycoproteins were found mainly on the apical surface and contained sialomucin. A deviated differentiation into an absorptive cell could form an intracytoplasmic cyst filled with an aggregation of glycocalyces, thus becoming another type of signet ring cell.

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