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Peptides of the neuro intermediary lobe of rat pituitary stimulate growth hormone secretion in vitro



Peptides of the neuro intermediary lobe of rat pituitary stimulate growth hormone secretion in vitro



Molecular & Cellular Endocrinology 32(1): 47-56



.alpha.-MSH and other fragments of ACTH are potent stimulators of GH [growth hormone] release in vivo. The action of such peptides and of extracts of the neurointermediary lobe (NIL) of rat pituitary, a source of endogenous MSH-related peptides, on GH release was investigated in vitro. Peptides with the core sequence of .alpha.-MSH stimulate GH secretion by primary cultures of rat anterior pituitary cells; however, both the absolute and the relative potencies of these peptides exclude the involvement of melanotropic receptors comparable in specificity to the extrapituitary receptors for these hormones. Extracts of the NIL of rat pituitary stimulate GH release in vitro and the bulk of the releasing activity can be attributed to 1 (or several) factors with an apparent mass of .apprx. 10,000 MW that can be partially purified by high performance liquid chromatography. The active principle appears to be distinct from both .beta.-lipotropin and the human pancreatic growth hormone releasing factor. Thus, while rat NIL contains GH-releasing activity that can be demonstrated in vitro, a direct link to the potent action of MSH-related peptides on GH release in vivo cannot be established, and the action of these peptides in vivo must therefore rely on mechanisms which are not expressed in the in vitro system.

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Accession: 006080110

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