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Phage internal proteins 6. interaction of bacterio phage t 4 internal proteins with t 4 dna in vivo and in vitro



Phage internal proteins 6. interaction of bacterio phage t 4 internal proteins with t 4 dna in vivo and in vitro



Revista Brasileira de Pesquisas Medicas e Biologicas 13(1-3): 41-46



Internal proteins are basic proteins bound to the DNA of T4 coliphage. Centrifugation in sucrose gradients was employed to isolate DNA-internal proteins complexes formed in vitro. Polyacrylamide gel electrophoresis of radioactive internal proteins in the presence of sodium dodecyl sulfate followed by autoradiography of the gels was used to identify the DNA-bound proteins and to determine their respective abundance in these complexes and in the bacteriophage head. The internal proteins bound to the phage DNA (in vitro experiments) at the same ratio as that of internal proteins associated with the viral DNA within the phage head (in vivo binding).

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