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Phage typing of Staphylococcus epidermidis

Journal of Clinical Microbiology 3(5): 519-523

Phage typing of Staphylococcus epidermidis

Thirteen phages [native] were isolated from lysogenic cultures of S. epidermidis from a clinical laboratory and used to type 223 clinical [human] isolates of this organism. The 18 phages isolated in The Netherlands were used to type these same cultures. No correlation was observed between phage type, biotype or clinical source of isolation. At phage concentrations of 100 times the routine test dilution, 35.0% of the cultures were typable with native phages and 21.5% were typable with the phages from The Netherlands. When only cultures in biotype 1 were considered, 43.3 and 24.1% of 141 cultures were typable with native phages and those from The Netherlands, respectively. The lytic reactions obtained with native phages were generally stronger and easier to read and the lytic patterns were, almost invariably, shorter. The typability of untypable cultures was increased 12.0% by incubation at C prior to phage typing and 20% by heat shock ( C for 5 min) prior to typing. Phage typing 5 subcultures of 20 typable cultures on 5 successive days showed that the lytic patterns were reproducible. The present status of phage typing S. epidermidis and the work needed to obtain a set of typing phages for epidemiological studies of infections by this organism are discussed.

Accession: 006092295

PMID: 132457

Related references

Ferreirós, C.M.; Souto, M.J.; Criado, M.T.; Suárez, P., 1991: Phage typing and phage induction in carrier and invasive Staphylococcus epidermidis isolates. Fifty-nine Staphylococcus epidermidis isolates (30 carrier and 29 invasive) were phage typed using the phage set developed by Pulverer. Poor results were obtained as only 24% of the invasive and 27% of the carrier isolates were typable, with no di...

Hoover D.L.; Khan J.A.; Parisi J.T.; Ellis R.E.; Burns R.P.; Ide C.H., 1986: Comparative epidemiology of staphylococcus epidermidis in chronic blepharitis patients and controls using phage typing antibiograms api staph ident typing and plasmid analysis. Investigative Ophthalmology & Visual Science 27(3 SUPPL): 36

Parisi J.T.; Talbot H.W.Jr; Skahan J.M., 1978: Development of a phage typing set for staphylococcus epidermidis in the usa. Zentralblatt fuer Bakteriologie Parasitenkunde Infektionskrankheiten und Hygiene Erste Abteilung Originale Reihe A Medizinische Mikrobiologie und Parasitologie 241(1): 60-67

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Goto, S.; Niwa, C.; Kuwahara, S., 1965: Transduction of drug-resistances in Staphylococcus. II. Transduction of chloramphenicol-resistance in both Staphylococcus aureus and Staphylococcus epidermidis by typing phage 80. Japanese Journal of Microbiology 9(1): 15-19

Parisi, J.T.; Talbot, H.W.; Skahan, J.M., 1978: Development of a phage typing set for Staphylococcus epidermidis in the United States. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie und Parasitologie 241(1): 60-67

Kryński, S.; Becla, E.; Samet, A.; Nowicki, B.; Wielgosz, A., 1975: Usefulness of phage typing for identification of Staphylococcus Epidermidis and Micrococcus strains. Archivum Immunologiae et Therapiae Experimentalis 23(5): 645-650

Blouse, L.; Kolonel, L.N.; Watkins, C.A.; Atherton, J.M., 1975: Efficacy of phage typing epidemiologically related Staphylococcus epidermidis strains. A total of 118 epidemiologically related Staphylococcus epidermidis strains from hospital patients, staff, and fomites were examined with a provisional set of 18 typing phages. Seventy (59.3%) of these strains were typed using phage concentrations...

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