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Phagocytosis of Giardia muris by macrophages in Peyer's patch epithelium in mice






Infection and Immunity 33(2): 591-601

Phagocytosis of Giardia muris by macrophages in Peyer's patch epithelium in mice

No mechanism for the initiation of immunological clearance of Giardia from the mammalian intestinal tract has been identified. In normal and nude mice experimentally infected with G. muris, we examined antigen-sampling epithelium over Peyer's patch follicles by electron microscopy for evidence of interaction between G. muris and lymphoid cells. Invading G. muris were found in the epithelium near dying or desquamating columnar cells. Macrophages beneath the basal lamina extended pseudopods into the epithelium, trapping invading G. muris and enclosing them in phagolysosomes. In normal mice, which clear G. muris in 4 to 6 weeks, macrophages containing digested G. muris were surrounded by rosettes of lymphoblasts in the epithelium. In nude mice deficient in lymphocytes, there was apparent hyperplasia of macrophages, which filled the follicle domes, resulting in more frequent entrapment of G. muris but no contact between macrophages and lymphoblasts in the epithelium. In nude mice, which require 6 months to control G. muris infection, lymphoblast contact with macrophages containing distinctive microtubular remnants of G. muris was only identified in the follicle dome. This close physical association of lymphoblasts and macrophages containing G. muris remnants suggests that this macrophage activity represents intraepithelial antigen processing as well as a defense against the effects of the uncontrolled entrance of microorganisms and other antigenic particles into Peyer's patch lymphoid follicles.


Accession: 006092707

PMID: 7275318



Related references

Owen, R.L.; Allen, L.; Stevens, D.P., 1979: Antigen processing in experimental giardiasis: phagocytosis of Giardia by macrophages in Peyer's patch epithelium. Journal of Cell Biology 83(2, Pt. 2): 128a

Carlson, J.R.; Heyworth, M.F.; Owen, R.L., 1986: Response of peyer's patch lymphocyte subsets to giardia muris infection in balb c mice 2 b cell subsets enteric antigen exposure is associated with immunoglobulin isotype switching by peyer's patch b cells. The aim of this study was to quantify the response of Peyer's patch B cells, surface IgA-bearing (sIgA) B cells, and surface IgM-bearing (sIgM) B cells to Giardia muris infection. Following infection of a cohort of immunocompetent BALB/c mice...

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Djamiatun, K.; Faubert, G.M., 1998: Exogenous cytokines released by spleen and Peyer's patch cells removed from mice infected with Giardia muris. The role that T and B lymphocytes play in the clearance of Giardia muris in the mouse model is well known, but the cytokines produced by CD4+ T cells in response to Giardia antigenic stimulation are unknown. In this study, we have determined how G...

Djamiatun, K.; Faubert, G.M., 1998: Exogenous cytokines released by spleen and Peyer's patch cells removed from mice infected with Giardia muris. The role that T and B lymphocytes play in the clearance of Giardia muris in the mouse model is well known, but the cytokines produced by CD4+ T cells in response to Giardia antigenic stimulation are unknown. In this study, we have determined how G...

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Hill, D.R., 1990: Lymphocyte proliferation in Peyer's patches of Giardia muris-infected mice. Gastrointestinal immune events in giardiasis are important in controlling infection. In this study, Peyer's patch lymphocytes from mice infected with Giardia muris developed specific, proliferative responses to G. muris antigen. This prolifer...

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