EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Phagocytosis of Staphylococcus aureus by bovine mammary gland macrophages and intracellular protection from antibiotic action in vitro and in vivo


Journal of Dairy Research 51(4): 513-523
Phagocytosis of Staphylococcus aureus by bovine mammary gland macrophages and intracellular protection from antibiotic action in vitro and in vivo
Macrophages isolated from the involuted bovine mammary gland were cultured in vitro. Phagocytosis of opsonized Staphylococcus aureus occurred rapidly, but intracellular killing of bacteria was slow. Many intracellular staphylococci survived for up to 4 d exposure to extracellular cloxacillin and emerged from within the macrophages to multiply extracellularly when the antibiotic was inactivated. Rifampicin was significantly more efficient than cloxacillin in killing intracellular S. aureus after 18 h incubation, but it too failed to sterilize the cultures within 3 d. Staphylococci, which had remained viable within macrophages during 20 h incubation with extracellular cloxacillin, showed an increased sensitivity to dilute lysostaphin on subsequent exposure. A 3 d course of intramammary therapy with cloxacillin, commencing simultaneously with an infecting inoculum of approximately 10(8) colony forming units (c.f.u.) S. aureus, apparently eliminated the infection from one quarter of the udders of each of three lactating cows, but bacteria were re-isolated from two cows after a delay of several days. However, when other quarters of the same cows were infected with approximately 10(8) c.f.u. S. aureus which had been phagocytosed by autologous mammary macrophages, similar simultaneous antibiotic therapy failed to affect these infections. The in vitro and in vivo findings indicate the significance of intracellular survival of S. aureus as a factor contributing to failure of antibiotic therapy.


Accession: 006092794

PMID: 6569065

DOI: 10.1017/s0022029900032830



Related references

The effect of local immunization of the mammary gland on phagocytosis and intracellular kill of Staphylococcus aureus. 1977

Effect of local immunization of the mammary gland on phagocytosis and intracellular kill of Staphylococcus aureus by polymorphonuclear neutrophils. American Journal of Veterinary Research 41(9): 1427-1431, 1980

Antibiotic treatment of experimental Staphylococcus aureus infections of the bovine mammary gland. Canadian Journal of Comparative Medicine 38(4): 411-416, 1974

Evaluation of antibiotic effectiveness against Staphylococcus aureus surviving within the bovine mammary gland macrophage. Journal of Antimicrobial ChemoTherapy 21(6): 773-786, 1988

Phagocytosis and intracellular killing of Staphylococcus aureus by bovine blood polymorphonuclear leukocytes after migration in vitro. Zentralblatt für Veterinarmedizin. Reihe B. Journal of Veterinary Medicine. Series B 36(2): 154-156, 1989

In vitro adherence of Staphylococcus aureus to bovine mammary gland epithelial cells. Acta Veterinaria Scandinavica 22(1): 99-108, 1981

Effect of Staphylococcus aureus and Streptococcus uberis on apoptosis of bovine mammary gland neutrophils in vitro. Veterinarni Medicina 50(1): 11-23, 2005

In vitro and in vivo inactivation of penicillin by penicillinase from Staphylococcus aureus of bovine mammary origin. Kerala Journal of Veterinary Science 12(1): 31-35, 1981

I. The recovery of small numbers of Staphylococcus aureus infused into the bovine teat cistern. II. The response of the bovine mammary gland to an infusion of staphylococci. III. The effect of inoculating the bovine teat duct with small numbers of Staphylococcus aureus. J. Dairy Res, 32: 157-162, 163-170 & 171-179, 1965

Phagocytosis of Staphylococcus aureus by somatic cells in dry cow secretion of mammary gland. Acta Microbiologica Polonica 38(3-4): 271-283, 1989