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Pharbitin content in pharbitis nil

Reports of Faculty of Science Shizuoka University 21: 77-88

Pharbitin content in pharbitis nil

Fully matured seeds of Pharbitis nil, Violet, contained about 3% (w/w) resin glycoside "Pharbitin". We also investigated the pharbitin content in three seed parts (seed coat, cotyledons and hypocotyl with radicle), cotyledon giant cells, leaves, and open corollas with five stamens. In the seed, a pair of cotyledons stored about 94% of seed pharbitin. In the cotyledon, many giant cells (ca. 100 .mu.m in diameter) were observed among normal-sized mesophyll cells (isolated protoplasts, 13-25 .mu.m in diameter). Those giant cells were isolated enzymatically from the cotyledon, and their pharbitin was extracted. The pharbitin content in giant cells was about 70% of that in the cotyledon. Thus, the giant cells were major storage sites of seed pharbitin. At the early stage of seed formation, 10 to 11 days after anthesis, the giant cells initiated in immature cotyledons, and reached nearly to their final sizes 20 days after anthesis. Afterwards, their pharbitin content rapidly increased until seed maturation. In growing seedlings, the pharbitin content in a pair of cotyledons suddenly decreased in a day from four to five days after H2SO4-treatment (the time of cotyledon-pair unfolding), and did not decrease from five to ten days. In foliage leaves and open corollas with five stamens, pharbitin was also present at a relatively low level.

Accession: 006092957

Related references

Kawasaki T.; Okabe H.; Nakatsuka I., 1971: Studies on resin glycosides part 1 reinvestigation of the components of pharbitin a resin glycoside of the seeds of pharbitis nil d. Chemical & Pharmaceutical Bulletin (Tokyo) 19(6): 1144-1149

Ono, M.; Takigawa, A.; Mineno, T.; Yoshimitsu, H.; Nohara, T.; Ikeda, T.; Fukuda-Teramachi, E.; Noda, N.; Miyahara, K., 2010: Acylated glycosides of hydroxy fatty acid methyl esters generated from the crude resin glycoside (pharbitin) of seeds of Pharbitis nil by treatment with indium(III) chloride in methanol. Treatment of the crude ether-insoluble resin glycoside (convolvulin) from seeds of Pharbitis nil (Pharbitis Semen), called pharbitin, with indium(III) chloride in methanol provided seven oligoglycosides of hydroxy fatty acid methyl esters partiall...

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Yoneda, Y., 1978: Studies on interspecific hybrid in pharbitis part 4 genetic analysis of peroxidase isozymes in pharbitis nil and pharbitis purpurea hybrid. The interspecific hybrids between P. nil Choisy and P. purpurea Roth were very low in fertility or completely sterile. F2 segregations were studied on the cathodal peroxidase isozymes. P. purpurea has 3C isozyme and P. nil has 2C (Africa) and 1C (...

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Zeevaart, J.A., 1966: Reduction of the Gibberellin Content of Pharbitis Seeds by CCC and After-Effects in the Progeny. Plants of Pharbitis nil were treated with the growth retardant (2-chloroethyl) trimethylammonium chloride (CCC) shortly before and after anthesis. Fresh and dry weight of immature seeds were not affected by the CCC treatment.The level of gibberell...

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Ogawa, Y., 1965: Changes in the content of gibberellin-like substances of seeds and seedlings in the tall and dwarf varieties of Pharbitis nil Chois. The amount of the gibberellin-like substances in the seeds of the tall variety Tendan increased during the ripening process and reached a maximum on the 20th day after anthesis. The amount of these substances in the seeds of the dwarf variety Kida...

Stiles, J.I.J. ; Davies, P.J., 1976: Qualitative analysis by iso electric focusing of the protein content of pharbitis nil apices and cotyledons during floral induction. No differences were found in the protein patterns produced by apical tissue with and without floral induction. Cotyledons, however, repeatedly showed the distinct loss of a single protein band on floral induction.