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Pharbitin content in pharbitis nil






Reports of Faculty of Science Shizuoka University 21: 77-88

Pharbitin content in pharbitis nil

Fully matured seeds of Pharbitis nil, Violet, contained about 3% (w/w) resin glycoside "Pharbitin". We also investigated the pharbitin content in three seed parts (seed coat, cotyledons and hypocotyl with radicle), cotyledon giant cells, leaves, and open corollas with five stamens. In the seed, a pair of cotyledons stored about 94% of seed pharbitin. In the cotyledon, many giant cells (ca. 100 .mu.m in diameter) were observed among normal-sized mesophyll cells (isolated protoplasts, 13-25 .mu.m in diameter). Those giant cells were isolated enzymatically from the cotyledon, and their pharbitin was extracted. The pharbitin content in giant cells was about 70% of that in the cotyledon. Thus, the giant cells were major storage sites of seed pharbitin. At the early stage of seed formation, 10 to 11 days after anthesis, the giant cells initiated in immature cotyledons, and reached nearly to their final sizes 20 days after anthesis. Afterwards, their pharbitin content rapidly increased until seed maturation. In growing seedlings, the pharbitin content in a pair of cotyledons suddenly decreased in a day from four to five days after H2SO4-treatment (the time of cotyledon-pair unfolding), and did not decrease from five to ten days. In foliage leaves and open corollas with five stamens, pharbitin was also present at a relatively low level.


Accession: 006092957



Related references

Kawasaki T.; Okabe H.; Nakatsuka I., 1971: Studies on resin glycosides part 1 reinvestigation of the components of pharbitin a resin glycoside of the seeds of pharbitis nil d. Chemical & Pharmaceutical Bulletin (Tokyo) 19(6): 1144-1149

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