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Pharmacological and physiological properties of benzodiazepine binding sites in rodent brown adipose tissue



Pharmacological and physiological properties of benzodiazepine binding sites in rodent brown adipose tissue



Comparative Biochemistry & Physiology C Pharmacology Toxicology & Endocrinology 77(2): 339-344



[3H]Diazepam and [3H]Ro5-4864 [a diazepam analog] were used as ligands to identify and characterize peripheral-type benzodiazepine binding sites in mouse [Mus musculus] and rat [Rattus norvegicus] brown adipose tissue (BAT) membranes. [3H]Diazepam and [3H]Ro5-4864 binding sites in BAT are pharmacologically similar to peripheral-type benzodiazepine binding sites in other tissues. Stimulators of central-type benzodiazepine receptors had no effect on or inhibited ligand binding to BAT membranes. Brown adipose tissue benzodiazepine binding sites are highly localized to mitochondrial-containing subcellular fractions. These binding sites decrease with age in BAT from Fischer 344 rats. Stimulation of BAT thermogenesis in mice with 1-norepinephrine led to a decrease in [3H]Ro5-4864 binding in the tissue.

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Accession: 006097051

Download citation: RISBibTeXText

PMID: 6144440

DOI: 10.1016/0742-8413(84)90025-2


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