Phorbol ester treated chronic B lymphocytic leukaemia cells induce autologous T cell proliferation without generation of cytotoxic T cells

Kabelitz, D.; Tötterman, T.H.; Nilsson, K.; Gidlund, M.

Clinical and Experimental Immunology 57(2): 461-466


ISSN/ISBN: 0009-9104
PMID: 6235991
Accession: 006103643

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The ability of the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) to modify the stimulatory capacity of leukemic B cells in autologous mixed leukocyte reactions (AMLR) was studied. T lymphocytes from patients with chronic lymphocytic leukemia (CLL) did not proliferate when stimulated with untreated autologous non-T cells. In contrast, vigorous 3H-thymidine incorporation was seen in 6 of 8 cases when CLL stimulator cells were pre-incubated for 3 days with TPA. No cytotoxic T cells against TPA treated or untreated autologous target cells were generated in such AMLR. Enhancement of AMLR stimulatory capacity may be among the characteristic features of TPA driven in vitro differentiation of CLL cells.