+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Phospho lipid pools in insect muscle labeling by phosphorus 32 in sub cellular fractions in vivo

Phospho lipid pools in insect muscle labeling by phosphorus 32 in sub cellular fractions in vivo

Physiologia Bohemoslovaca 31(4): 289-296

The time course of labeled phospholipids (32P), followed for 14 days in vivo [males Periplaneta americana], shows that there is a fraction of PC (phosphatidylcholine) and PE (phosphatidylethanolamine) in mitochondria which exchanges phosphate very slowly; this fraction comprises .apprx. 25% of the total mitochondrial PC and .apprx. 50% of PE. In muscles, followed during maturation, these fractions were smaller. PI (phosphatidylinositol) isotopic equilibrium is established rapidly between the mitochondria and microsomal fraction. During anaerobiosis the labeling of microsomal PC and PE is slowed down, yet labeling of the mitochondrial phospholipids continues at a rate suggesting that a transport from microsomal membranes to mitochondria takes place in vivo. In the 105,000 .times. g supernatant small amounts of phospholipids were found, the specific activity of which was (for PC and PE) higher than that of microsomes after short incorporation times. A tentative scheme of the intracellular organization of phospholipid metabolism in insect flight muscle is being proposed.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 006105218

Download citation: RISBibTeXText

Related references

Phospholid pools in insect muscle: labelling by 32 P in subcellular fractions in vivo. Physiologia Bohemoslovaca 314: 289-296, 1982

Phospho lipid composition of porcine muscle and its sub cellular fractions. Journal of Animal Science 31(1): 180, 1970

Characterization of phospho lipids and localization of some phospho lipid synthetic and sub cellular marker enzymes in sub cellular fractions from rabbit lung. Biochimica et Biophysica Acta 666(1): 47-57, 1981

The effect of di phenyl guanidine on the in vivo incorporation of phosphorus 32 ortho phosphate in phospho lipid fractions of the liver and blood serum of rats. Farmakologiya i Toksikologiya 36(4): 444-446, 1973

Labeling of phospho lipids in the surfactant and sub cellular fractions of rabbit lung. American Review of Respiratory Disease 117(4 Part 2): 353, 1978

Effect of dietary lipid on chlorpromazine depression of phosphorus 32 labeling of rat brain phospho lipids. Life Sciences Part I Physiology & Pharmacology 8(3 Part 1): 215-223, 1969

Phospho lipid synthesis in sub cellular fractions of hormone treated rat liver. Federation Proceedings 32(3 Part 1): 601, 1973

Cholinergic stimulations of phosphorus 32 labeling of phospho lipids in rabbit iris muscle. Bulletin of the Georgia Academy of Science 33(2): 95, 1975

The labeling of phospho lipid with ortho phosphate phosphorus 32 as influenced by procedures which alter plasma cholesterol concentration. Proceedings of the Society for Experimental Biology & Medicine 137(2): 516-522, 1971

Effect of acth on protein and phospho lipid metabolism in sub cellular fractions of adrenal glands. Journal of Biochemistry 71(4): 615-623, 1972

Effect of dexamethasone on the phospho lipid content in sub cellular fractions of tonic and tetanic muscles. Acta Physiologica Academiae Scientiarum Hungaricae 50(2): 173-178, 1977

Thin layer chromatographic methods to isolate phosphorus 32 labeled 5 phospho ribosyl alpha 1 pyro phosphate determination of cellular 5 phospho ribosyl alpha 1 pyro phosphate pools and assay of 5 phospho ribosyl alpha 1 pyro phosphate synthetase activity. Analytical Biochemistry 98(2): 254-263, 1979

Effect of organo phosphorus cholin esterase inhibitors on metabolism of individual phospho lipid fractions in the rat brain. Bulletin of Experimental Biology & Medicine 74(11): 1398-1401, 1972, 1973

Fatty acid incorporation into cholesteryl ester and phospho lipid by arterial sub cellular fractions of swine. Proceedings of the Society for Experimental Biology and Medicine 155(1): 8-12, 1977

Phospho lipid metabolism in de nervated insect muscle. Physiologia Bohemoslovaca 31(3): 274-275, 1982