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Phospho lipid pools in insect muscle labeling by phosphorus 32 in sub cellular fractions in vivo



Phospho lipid pools in insect muscle labeling by phosphorus 32 in sub cellular fractions in vivo



Physiologia Bohemoslovaca 31(4): 289-296



The time course of labeled phospholipids (32P), followed for 14 days in vivo [males Periplaneta americana], shows that there is a fraction of PC (phosphatidylcholine) and PE (phosphatidylethanolamine) in mitochondria which exchanges phosphate very slowly; this fraction comprises .apprx. 25% of the total mitochondrial PC and .apprx. 50% of PE. In muscles, followed during maturation, these fractions were smaller. PI (phosphatidylinositol) isotopic equilibrium is established rapidly between the mitochondria and microsomal fraction. During anaerobiosis the labeling of microsomal PC and PE is slowed down, yet labeling of the mitochondrial phospholipids continues at a rate suggesting that a transport from microsomal membranes to mitochondria takes place in vivo. In the 105,000 .times. g supernatant small amounts of phospholipids were found, the specific activity of which was (for PC and PE) higher than that of microsomes after short incorporation times. A tentative scheme of the intracellular organization of phospholipid metabolism in insect flight muscle is being proposed.

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Accession: 006105218

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