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Photo control of plastein synthesis by alpha chymotrypsin spiropyran gel



Photo control of plastein synthesis by alpha chymotrypsin spiropyran gel



Journal of Molecular Catalysis 18(2): 135-140



Agarose gel was mofified with .beta.-1-[3,3-dimethyl-6'-nitrospiro-(indoline-2,2'-2H-benzopyran)] propionic anhydride. .alpha.-Chymotrypsin was immobilized on the modified agarose gel. The immobilized .alpha.-chymotrypsin showed a reverse photochromism. The photoresponsive enzyme was applied to the plastein reaction, and plastein was produced by the immobilized .alpha.-chymotrypsin. The yield of plastein synthesized under visible light was higher than that synthesized in the dark. Enzymatic properties of the modified .alpha.-chymotrypsin were also investigated. [The plastein reaction is useful in the production of food proteins].

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Accession: 006108488

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