Photo electric effects in bi layer membranes electrometers and voltage clamps compared/

Huebner, J.S.; Arrieta, R.T.; Arrieta, I.C.; Pachori, P.M.

Photochemistry and Photobiology 39(2): 191-198


ISSN/ISBN: 0031-8655
Accession: 006108617

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Laser flash induced voltage transients recorded with high speed electrometers are compared to signals generated by voltage clamps, using chromophores which generate photoelectric effects in membranes by different mechanisms and with time constants that varied from < 100 ns to > 1 s. The chromophores used were: the azo dye, 3,3'-bis-(.alpha.-(trimethylammonium)methyl)azobenzene-bromide; the cyanine dye, 3,3'-dimethyl-2,2'-oxacarbocyanine-chloride; the xanthene dye, 2',4',5',7'-tetraiodofluorescein or erythrosin; and chlorophyll a. A scheme for classifying photoelectric effects is presented. Voltage clamp signals are functions of both apparatus and photo-current time constants. They vary with aqueous solution salt concentration, and usually mask kinetic information that may be obtained with electrometers.