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Photochemical cross linking of the gene 5 protein fd dna complex from phage fd infected cells



Photochemical cross linking of the gene 5 protein fd dna complex from phage fd infected cells



Journal of Biological Chemistry 257(3): 1462-1467



The gene 5 protein.cntdot.fd DNA complex, which is an intermediate in the life cycle of fd bacteriophage, was isolated from fd-infected [Escherichia coli] cells. After UV irradiation, .apprx. 10% of the gene 5 protein was covalently linked to fd DNA via the SH group of the single cysteine residue at position 33 in the gene 5 protein. When intact, infected cells were 1st irradiated with UV light and the gene 5 protein.cntdot.DNA complex subsequently isolated, < 1% of the bound protein in the complex was cross-linked. While these results agree with previous findings based on irradiation of the gene 5 protein.cntdot.fd DNA complex formed from purified gene 5 protein and fd DNA, they differ from the high yields of cross-linked gene 5 protein.cntdot.fd DNA complex after UV irradiation of intact cells.

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Accession: 006110006

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