EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Photochemical generation of superoxide radical and the cytotoxicity of phthalocyanines






International Journal of Radiation Biology & Related Studies in Physics Chemistry & Medicine 48(5): 837-846

Photochemical generation of superoxide radical and the cytotoxicity of phthalocyanines

The effect of the central mental atom on the photodynamic activity of phthalocyanine dyes has been estimated by cytotoxicity to cultured Chinese hamster cells. Chloroaluminium phthalocyanine, followed by the Zn- derivative, were found to be the only active dyes. In parallel it was found that visible light (615 .+-. 10 nm) excitation of phthalocyanines dissolved in dimethylsulphoxide in the presence of oxygen generates superoxide radical anion. O2- radicals were spin-trapped with 5,5-dimethyl-1-pyrroline-1-oxide (DMPO) and identified by electron spin resonance. The quantum yields for O2- generation range from 10-5 (Zn-phthalocyanine) to 4.2 .times. 10-4 (Ga-phthalocyanine). The efficiency of generating O2- was apparnetly uncorrelated with the phototoxicity of the same dyes. Furthermore, the biological photodamage could be inhibited by the addition of superoxide dismutase. It is concluded that O2- is involved very little, if at all, in the phthalocyanine-induced photon-killing of mammalian cells.

Accession: 006110066

PMID: 2997064

DOI: 10.1080/09553008514551931

Download PDF Full Text: Photochemical generation of superoxide radical and the cytotoxicity of phthalocyanines



Related references

Draper, W.M.; Crosby, D.G., 1983: Photochemical generation of superoxide radical anion in water. Journal of Agricultural and Food Chemistry 31: 4-7

Zhang, X.F.; Xu, H.J.n; Chen, D.W.n, 1994: Substituted phthalocyanine-sensitized photochemical generation of superoxide anion radical. The quantum yield of phthalocyanine-photosensitized generation of superoxide anion radical (O-2-) was measured by the electron spin resonance (ESR) technique. From the redox potential data, it is concluded that O-2- is formed by electron transfer...

Draper, W.; Crosby, D., 1983: Photochemical generation of superoxide radical anion in water Sensitized photooxidation of pesticides in water. Journal of agricultural and food chemistry 31(4): 734-737

Kato, M.; Minakami, H.; Kuroiwa, M.; Kobayashi, Y.; Oshima, S.; Kozawa, K.; Morikawa, A.; Kimura, H., 2003: Superoxide radical generation and Mn- and Cu-Zn superoxide dismutases activities in human leukemic cells. Mn- and Cu-Zn superoxide dismutase (SOD) activities and generation of superoxide radicals (O(2) (-)) were assessed in leukemic cells from 10 patients with acute myeloid or monocytic leukemia (AML) and 10 patients with acute lymphoblastic leukemia...

Sawada, M.; Carlson, J.C., 1994: LH stimulation of superoxide radical generation and the effect of superoxide dismutase on steroidogenesis. Biology of Reproduction 50(SUPPL 1): 144

Navari-Izzo, F.; Pinzino, C.; Quartacci, M.F.; Sgherri, C.L., 2000: Superoxide and hydroxyl radical generation, and superoxide dismutase in PSII membrane fragments from wheat. Illumination of photosystem II (PSII) membrane fragments of wheat (Triticum durum Desf. cv. Adamello) gave rise to both O2*- and *OH radicals adducts of the novel spin trap 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide (DEPMPO). With time, *...

Fujita, Y., 1986: Generation of superoxide radical in behcet's disease and the effects of superoxide dismutase on experimental uveitis. In order to clarify neutrophil dysfunction in Behcet's disease, generation of superoxide radicals from neutrophils in patients with this disease was measured. As a result, superoxide production of neutrophils in patients with Behcet's di...

Navari Izzo, F.; Pinzino, C.; Quartacci, M.F.; Sgherri, C.L.M., 1999: Superoxide and hydroxyl radical generation, and superoxide dismutase in PSII membrane fragments from wheat. Illumination of photosystem II (PSII) membrane fragments of wheat (Triticum durum cv. Adamello) gave rise to both O2- and OH radicals adducts of the novel spin trap 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide (DEPMPO). With time, OH became...

Verma S.; Kumar G.P.; Laloraya M.; Singh A.; Nivsarkar M.; Bharti S., 1991: A possible role of superoxide anion radical and superoxide dismutase in tyrosine iodination novel and alternate hydrogen peroxide generation machinery. Journal of Cell Biology 115(3 PART 2): 77A

Evans C.H.; Scaiano J.C., 1990: Photochemical generation of radical cations from alpha terthienyl and related thiophenes kinetic behavior and magnetic field effects on radical ion pairs in micellar solution. The photochemistry of .alpha.-terthienyl (.alpha.T) and related compounds has been examined in homogeneous solution and in anionic micelles in the presence of electron acceptors. The absorption spectra of the radical cations from four thiophenic s...