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Photochemically active chloroplasts from spruce picea abies


Photosynthetica (Prague) 12(3): 323-327
Photochemically active chloroplasts from spruce picea abies
A procedure for the isolation of photochemically active chloroplasts from spruce needles is described. With this method, chloroplasts were prepared at the different structural stages during the year and ferricyanide dependent O2 evolution and photophosphorylation and phenazine methosulfate (PMS)+ mediated photophosphorylation were measured. With chloroplasts from fully developed needles both ferricyanide dependent photoreactions (Hill reaction and non-cyclic photophosphorylation) reached maximum rates in early summer (150 .mu.mol O2 and 120 .mu.mol ATP, mg-1 (chl [chlorophyll]) h-1, respectively) and were lowest during winter (40 .mu.mol O2 and 20 .mu.mol ATP, respectively). The setback of rates in winter is in agreement with the disintegration of the thylakoid system and with the known low capability for CO2 fixation during the cold season. PMS [phenazine methosulfate] catalyzed cyclic photophosphorylation reached maximum rates in winter (80-160 .mu.mol ATP mg-1 (chl) h-1, depending on the age of the needles), but was hard to detect in summer.


Accession: 006110253



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