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Photodynamic damage to protoplasts with a hematoporphyrin derivative

Photodynamic damage to protoplasts with a hematoporphyrin derivative

Plant Science (Shannon) 44(2): 139-144

Leaf cell protoplasts were isolated from Vicia faba L. The protoplasts originated from both epidermal, guard and mesophyll cells and had a variable content of chloroplasts. After incubation with hematoporphyrin derivative and a washing procedure, the cells became sensitive to near UV. Photodamage was manifested at the plasmalemma as 'blebs', extrusion of some of the cellular content and finally disintegration. Blebbing and cell lysis developed within less than 1 h after irradiation. The extent of the photodamage was dependent on both the concentration of porphyrin and the light fluence. Different cell types with different amounts of chloroplasts were equally sensitive to the photodynamic treatment.

Accession: 006110567

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DOI: 10.1016/0168-9452(86)90083-X

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