EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Photoinactivation sites of photosystem i in isolated chloroplasts


Plant & Cell Physiology 27(6): 961-968
Photoinactivation sites of photosystem i in isolated chloroplasts
Illumination of isolated spinach chloroplasts under aerobic conditions induced photoinactivation of PS I as measured by NADP+ photoreduction with 2,6-dichlorophenolindophenol and ascorbate as electron donors. The iron-sulfur centers X, B, and A also were decreased. The extent of photoinactivation of any of these centers or P700 as single components could not account for the loss of PS I activity. It was correlated, however, with the product of each fraction of the three iron-sulfur centers remaining intact. These results suggest a linear electron transfer sequence of the three centers. Incubation of chloroplasts with xanthine oxidase in the dark also led to decrease of PS I activity as well as of iron-sulfur centers. It is concluded that the main cause of photoinactivation of PS I is the destruction of the iron-sulfur centers by some species of active oxygen produced by illuminated class II chloroplasts.


Accession: 006110826



Related references

Photoinactivation of chloroplasts already inhibited on the oxidizing side of photosystem ii. Biochimica et Biophysica Acta 849(1): 104-111, 1986

Identification of S2 as the sensitive state to alkaline photoinactivation of photosystem II in chloroplasts. Biochimica et Biophysica Acta 461(1): 61-74, 1977

Wavelength-dependent photophosphorylation catalysed by photosystem 1 or photosystem 2 in isolated pea chloroplasts. Photosynthetica 13(4): 359-364, 1979

Photosystem II can control the activity of photosystem I in isolated chloroplasts, but the reverse situation exists in leaves. Biological Chemistry Hoppe-Seyler 375(SPEC SUPPL 1): S51, 1994

Effect of hydration level in primary bean leaves on the activity of photosystem i and photosystem ii in isolated chloroplasts. Biologia Plantarum (Prague) 18(6): 473-479, 1976

A current method for measuring the electron transport rate of photosystem I and photosystem II from samples of isolated chloroplasts, and comments on it. Journal of the Chinese Agricultural Chemical Society 35(2): 202-208, 1997

Photo induced changes of carotenoid composition in isolated pigment proteo lipid complexes of photosystem 1 and photosystem 2 of the chloroplasts. Soviet Plant Physiology 23(3): 386-392, 1976

Photo induced changes in carotenoid composition of the pigment protein lipid complexes of photosystem i and photosystem ii isolated from chloroplasts. Fiziologiya Rastenii (Moscow) 23(3): 452-459, 1976

In vitro synthesis and assembly of photosystem II core proteins. The D1 protein can be incorporated into photosystem II in isolated chloroplasts and thylakoids. Journal of Biological Chemistry 270(43): 25685-25695, 1995

Properties of photoreductions by photosystem I in isolated chloroplasts. and energy-conserving step in the photoreduction of benzoquinones by photosystem II in the presence of dibromothymoquinone. Biochimica et biophysica acta: 305 (1) 129-139, 1973