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Photolabelling of cholera toxin by NAD+

, : Photolabelling of cholera toxin by NAD+. Biochemical Journal 242(3): 927-930

When cholera toxin is incubated under u.v. light with NAD+ labelled in either the adenine or the nicotinamide moiety, radioactivity becomes covalently bound to the protein. The reaction is specific for cholera toxin, and is inhibited by excess unlabelled NAD+ or NAD analogues. Only the active A 1 chain of the toxin is labelled. The u.v.-absorption spectrum of the product is very similar to that of NAD+, and shows the same reaction with cyanide. The nature of the product is therefore different from that found when diphtheria toxin is photolabelled [Carroll & Collier (1984) Proc. Natl. Acad. Sci. U.S.A. 81, 3307-3311] in that the yield is lower, but both moieties of the NAD molecule become bound.

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Accession: 006110916

PMID: 3593286

DOI: 10.1042/bj2420927

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Related references

Wisnieski, B.J.; Bramhall, J.S., 1981: Photolabelling of cholera toxin subunits during membrane penetration. There has been much speculation about the mechanism by which cholera toxin exerts its effect on the cytoplasmic side of the membranes with which it interacts. After the pentamer of B subunits (5B) binds to membrane receptors, particularly the mono...

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