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Physiological studies on the formation of hairy root by the agrobacterium rhizogenes i. relationships between iaa content and morphological characteristics in carrot infected by agrobacterium rhizogenes


Physiological studies on the formation of hairy root by the agrobacterium rhizogenes i. relationships between iaa content and morphological characteristics in carrot infected by agrobacterium rhizogenes



Korean Journal of Botany 29(4): 275-284



Inoculation of carrot discs with Agrobacterium rhizogenes harboring Ri plasmid resulted in transformation of cells, as revealed by the tumors and hairy roots arising from them. Measurements of IAA content using HPLC indicate that it is higher in tumors and inoculated tissues than in uninoculated tissue. A lot of meristemoids and vessel elements formed in tumor tissue and the hairy roots differentiated from meristemoids. IAA content in tumor tissues is decreased with hairy root and vessel elements formation from them. Formation of wound callus in uninoculated tissues resulted on wound healing but no formation of vessel elements and hairy roots. Tumor tissues show continuous growing on hormone free medium.

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