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Picosecond fluorescence kinetics and energy transfer in chloroplasts and algae

Picosecond fluorescence kinetics and energy transfer in chloroplasts and algae

Biochimica et Biophysica Acta 680(2): 161-173

Single-photon timing with picosecond resolution is used to investigate the kinetics of the fluorescence [F] emission of chlorophyll a in chloroplasts from spinach and pea and in the algae Chlorella pyrenoidosa and Chlamydomonas reinhardii. The fluorescence decay is best described by 3 exponential components in all species. At low light intensity and with open reaction centers of Photosystem II (F0) lifetimes of .apprxeq. 100, 400 and 1100 ps for the 3 components were found. Closing the reaction centers by addition of 3-(3 ,4 -dichlorophenyl)-1,1-dimethylurea plus hydroxylamine and by increasing light intensity produces only minor changes in the almost constant fast- and medium-lifetime components; however, there is a dramatic increase in the yield of the slow component, by a factor of about 20, accompanied by only a modest increase in the lifetime to 2200 ps (Fmax). In good agreement with previous fluorescence lifetime measurements, an increase in the averaged lifetime of the 3 components from 0.5 to 2.0 ns was found, which is proportional to the 4-fold increase in the total fluorescence yield. Time-resolved results were inconsistent with models which are based on the proportionality between lifetime and yield and which involve a homogeneous origin of fluorescence that is sensitive to the state of the reaction centers. The variable part of the fluorescence, which is dominated by the slow phase probably reflects the kinetics of charge recombination in the reaction center, as proposed previously. The modest increase in lifetime of the slow phase indicates the presence of some energy transfer between photosynthetic units.

Accession: 006121723

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DOI: 10.1016/0005-2728(82)90007-x

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