Plant regeneration from callus culture in wheat triticum aestivum cultivar maris huntsman

Ahloowalia, B.S.

Crop Science 22(2): 405-410


ISSN/ISBN: 0011-183X
Accession: 006126462

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Plant regeneration was investigated from embryo-callus culture in bread wheat, T. aestivum L. Immature embryos of cultivar Maris Huntsman were cultured on half-strength Murashige and Skoog's medium (half-MS) supplemented with 0.5 mg 2,4-D 3.2 mg IAA and 1.0 mg kinetin [6-(furfurylamino)purine]/liter or without kinetin. On medium with 2,4-D, IAA and kinetin, 77% of the embryos produced a visible callus within 2 wk. On medium without kinetin, all embryos produced a callus. Calli were cloned and maintained for over 900 days by subculturing at 50 to 60 days interval on half-MS medium with 0.5 mg 2,4-D/liter but regeneration ceased after 680 days. Calli showed organogenesis within 120 days of subculture on half-MS medium with 0.5 mg 2,4-D and 1.0 mg zeatin [6-(4-hydroxy-3-methyl but-2-enylamino)aminopurine]/liter. Plantlets originated from callus by differentiation into shoot-primordia and bipolar structures. Plantlets produced roots on half-MS medium without growth regulators; however, 1.0 mg naphthaleneacetic acid/liter in the medium promoted rooting. Regenerated plants included variants for plant height, stem thickness, leaf size, spike shape, pollen fertility and seed set.