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Plasmodium falciparum in culture use of outdated erythrocytes and description of the candle jar method

Plasmodium falciparum in culture use of outdated erythrocytes and description of the candle jar method

Journal of Parasitology 63(5): 883-886

P. falciparum was cultured continuously in a simplified technique using plastic petri dishes in a candle jar. This method has the advantage of being readily adaptable to many experiments, especially those requiring numerous replicates. Experiments using this method allowed examination of some factors responsible for variations in parasite multiplication rates. Generally, fetal calf serum is not comparable to human serum, modifications to the RPMI 1640 medium are usually deleterious, and erythrocytes aged in citrated plasma are better for malarial cultures than freshly obtained cells; thus, erythrocytes outdated by blood-bank standards could provide a readily available supply of cells for large-scale production of malarial antigens.

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Accession: 006133199

Download citation: RISBibTeXText

PMID: 335035

DOI: 10.2307/3279900

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