EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Plasmodium falciparum in culture use of outdated erythrocytes and description of the candle jar method



Plasmodium falciparum in culture use of outdated erythrocytes and description of the candle jar method



Journal of Parasitology 63(5): 883-886



P. falciparum was cultured continuously in a simplified technique using plastic petri dishes in a candle jar. This method has the advantage of being readily adaptable to many experiments, especially those requiring numerous replicates. Experiments using this method allowed examination of some factors responsible for variations in parasite multiplication rates. Generally, fetal calf serum is not comparable to human serum, modifications to the RPMI 1640 medium are usually deleterious, and erythrocytes aged in citrated plasma are better for malarial cultures than freshly obtained cells; thus, erythrocytes outdated by blood-bank standards could provide a readily available supply of cells for large-scale production of malarial antigens.

(PDF same-day service: $19.90)

Accession: 006133199

Download citation: RISBibTeXText

PMID: 335035

DOI: 10.2307/3279900



Related references

A simple technique for in vitro culture of Plasmodium falciparum by the candle jar method. Transactions of the Royal Society of Tropical Medicine and Hygiene8(1): 137, 1984

Storage requirements for erythrocytes used to culture Plasmodium falciparum. Journal of Parasitology 69(1): 158-162, 1983

Detection of CD4+CD45RO+ T lymphocytes producing IL-4 in response to antigens on Plasmodium falciparum erythrocytes: an in vitro correlate of protective immunity induced with attenuated Plasmodium falciparum sporozoites. Cellular Immunology 180(2): 143-152, 1997

Concentration from continuous culture of erythrocytes infected with trophozoites and schizonts of Plasmodium falciparum. American Journal of Tropical Medicine and Hygiene 27(6): 1274-1276, 1978

Long term culture of Plasmodium falciparum in human erythrocytes with reduced K+ content. Transactions of the Royal Society of Tropical Medicine and Hygiene 80(6): 1007-1008, 1986

The influence of ABO group erythrocytes on the growth of Plasmodium falciparum in continuous in vitro culture. Journal of Parasitology and Parasitic Diseases 1(3): 141-143, 1983

Characterization of the humoral immune response in plasmodium falciparum malaria 1. estimation of antibodies to plasmodium falciparum or human erythrocytes by means of micro elisa enzyme linked immuno sorbent assay. Clinical and Experimental Immunology 54(1): 127-134, 1983

The effects of aspirin containing serum in the continuous culture of plasmodium falciparum infected human erythrocytes. Clinical Research: 381a, 1982

Stage-dependent alteration of negative charges of uninfected erythrocytes in Plasmodium falciparum culture. In Vitro Cellular and Developmental Biology 27A(8): 595-596, 1991

Micro in vitro chloroquine sensitivity test for Plasmodium falciparum without the use of candle jar. Indian Journal of Medical Research 76: 195-198, 1982