EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Plastein synthesis by alpha chymotrypsin immobilized on hydrophobic agarose gel



Plastein synthesis by alpha chymotrypsin immobilized on hydrophobic agarose gel



Journal of Molecular Catalysis 9(4): 445-451



.alpha.-Chymotrypsin was immobilized on hydrophobic agarose gels (octyl-Sepharose, phenyl-Sepharose). Native and immobilized .alpha.-chymotrypsin were employed for the plastein reaction. The yield of plastein from the immobilized .alpha.-chymotrypsin was higher than that using native .alpha.-chymotrypsin. The optimum pH value of native and immobilized .alpha.-chymotrypsin for plastein synthesis was 5.5. The immobilized .alpha.-chymotrypsin was stable under alkaline conditions. The optimum substrate concentration for the immobilized .alpha.-chymotrypsin was 25%. The immobilized .alpha.-chymotrypsin can be used repeatedly for plastein synthesis. The loss of catalytic activity was within 30% after 5 use cycles. The MW of the plastein synthesized was determined by the SDS[sodium dodecyl sulfate]-polyacrylamide disc-electrophoresis method. The MW of the plastein was from 13,000-30,000.

(PDF emailed within 1 workday: $29.90)

Accession: 006133355

Download citation: RISBibTeXText



Related references

Plastein synthesis with a chymotrypsin immobilized on chitin. Journal of the Science of Food and Agriculture 31(3): 273-278, 1980

Peptide synthesis in aqueous-organic solvent mixtures with alpha-chymotrypsin immobilized to tresyl chloride-activated agarose. Biotechnology and Bioengineering 26(10): 1146-1154, 1984

Modifications of some peptic alfalfa leaf protein peptides as a result of plastein reaction with immobilized alpha chymotrypsin. Zeitschrift fuer Lebensmittel-Untersuchung und -Forschung 175(4): 249-252, 1982

Plastein synthesis with alpha-chymotrypsin immobilised on chitin. Journal of the science of food and agriculture 31(3): 273-278, 1980

Alpha-Chymotrypsin in plastein synthesis Influence of water activity. Clark, D S , Estell, D A Annals of the New York Academy of Sciences; Enzyme engineering : 409-414, 1992

Photo control of plastein synthesis by alpha chymotrypsin spiropyran gel. Journal of Molecular Catalysis 18(2): 135-140, 1983

Probes for catalytic action of alpha-chymotrypsin in plastein synthesis. Agricultural and biological chemistry: 36 (9) 1595-1602, 1972

alpha-Chymotrypsin in plastein synthesis: influence of substrate concentration on enzyme activity. Biotechnology and Applied Biochemistry 14(2): 212-221, 1991

Plastein synthesis by the action of alpha chymotrypsin on a peptic hydrolysate of whey protein. Journal of Dairy Research 55(4): 547-550, 1988

Alpha chymotrypsin in plastein synthesis effect of hydroxylated additives on enzyme activity. Applied Biochemistry & Biotechnology 33(1): 51-65, 1992

Enzymatic attack on immobilized substrates. 1. The alpha-chymotrypsin-catalyzed hydrolysis of agarose-bound L-phenylalanine 4-nitroanilide. European Journal of Biochemistry 88(2): 445-452, 1978

Peptide synthesis by means of immobilized enzymes 1. immobilized alpha chymotrypsin ec 3.4.21.1. Monatshefte fuer Chemie 112(4): 469-482, 1981

Enzymatic attack on immobilized substrates part 1 the alpha chymotrypsin ec 3.4.21.1 catalyzed hydrolysis of agarose bound l phenyl alanine 4 nitro anilide. European Journal of Biochemistry 88(2): 445-452, 1978

A common size parameter for hydrophobic binding of inhibitors by alpha-chymotrypsin, alkylated alpha-chymotrypsin, and yeast alcohol dehydrogenase. Archives of Biochemistry and Biophysics 124(1): 530-534, 1968

Salt effects on plastein formation by alpha chymotrypsin. Agricultural & Biological Chemistry 39(6): 1207-1210, 1975