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Plate diffusion assay as a rapid method for dosimetry of mutagens



Plate diffusion assay as a rapid method for dosimetry of mutagens



Applied and Environmental Microbiology 38(6): 1127-1131



A method for determining mutagenic concentrations of chemicals by using an agar diffusion assay is presented. The method is based on the linear relationship between the amount of chemical placed at the center of the dish and the radius of the mutagenic zone. A brief theoretical discussion and experimental data confirming this relationship are given. Alkylating agents and mycotoxins were used to test the system. This method can be used to follow up decreased mutagenic potencies of solutions of unstable mutagens and to follow the production of mutagenic substances throughout fermentation. [Applicability of this method to the Ames test using Salmonella typhimurium tester strains with mammalian microsomal metabolic activation is discussed.].

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Accession: 006133675

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PMID: 393170



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