EurekaMag
+ Translate
+ Most Popular
Gaucher's disease;thirty-two years experience at Siriraj Hospital
A study of Macrobathra Meyrick from China (Lepidoptera, Cosmopterigidae)
First occurrence in ores of tetragonal chalcocite
Effects of trace element nutrition on sleep patterns in adult women
N.Z. range management guidelines. 2. Design of grazing management systems for tussock country
A case of lipoma of the esophagus
A revision of world Acanthosomatidae (Heteroptera: Pentatomidae): keys to and descriptions of subfamilies, tribes and genera, with designation of types
Life history of the coronate scyphozoan Linuche unguiculata (Swartz, 1788)
Perceptual restoration of obliterated sounds
Mutagenicity studies on two chromium(III) coordination compounds
The formation of the skeleton. I. Growth of a long bone. 1st appearance of a center of calcification
Leucopenia and abnormal liver function in travellers on malaria chemoprophylaxis
The joint commission: four key root causes loom large in sentinel event data
Treatment of vitiligo with topical 15% lactic acid solution in combination with ultra violet-A
Behaviour of dairy cows within three hours after feed supply: I. Influence of housing type and time elapsing after feed supply
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Management and control of patients with type 2 diabetes mellitus in Lebanon: results from the International Diabetes Management Practices Study (IDMPS)
The diet composition and nutritional knowledge of patients with anorexia nervosa
Physoporella croatica Herak, 1958 of the Slovak karst Anisian (Slovakia, the West Carpathians Mts.)
Bright lights, big noise. How effective are vehicle warning systems?
Ein Plesiosaurier-Rest mit Magensteinen aus mittlerem Lias von Quedlinburg
Incidence of Chlamydia trachomatis in patients with sterility
Monster soup: the microscope and Victorian fantasy
Preliminary tests with residual sprays against poultry lice
Duration of the life of plants in phylogeny

Pole cells of Drosophila melanogaster in culture. Normal metabolism, ultrastructure, and functional capabilities


Pole cells of Drosophila melanogaster in culture. Normal metabolism, ultrastructure, and functional capabilities



Developmental Biology 69(2): 451-465



ISSN/ISBN: 0012-1606

PMID: 108161

The metabolism, ultrastructure and function of mass-isolated pole cells were examined during short-term culture in vitro. In addition to demonstrating that these cells functioned normally in culture, a number of new features of embryonic pole cells were discovered. Cell populations isolated from Renografin density gradients were incubated in medium containing 3H-valine, uridine or thymidine. Although pole cells incorporated similar amounts of valine into protein as other embryonic cells throughout the 1st 6 h in culture, they began to synthesize RNA only after 2 h in culture. Approximately 30% of the pole cells synthesized DNA in vitro and this synthetic activity occurred largely during the 1st h of culture. An ultrastructural analysis of colcemid-treated cells showed that 10% of the pole cells divide shortly after placement in culture. During pole cell culture in vitro, polar granules and nuclear bodies fragment and disperse so that they are eventually not detected in these cells. These changes also occur during pole cell development in vivo. Among the fertile adults which were derived from embryos receiving transplantation of isolated pole cells before and after culture in vitro, 25-30% germ line mosaicism was obtained. These cells are able to follow their normal developmental program in vitro and are able to give rise to functional germ cells in vivo.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 006138061

Download citation: RISBibTeXText

Related references

Lineage analysis of transplanted individual cells in embryos of Drosophila melanogaster : III. Commitment and proliferative capabilities of pole cells and midgut progenitors. Roux's Archives of Developmental Biology: the Official Organ of the Edbo 195(8): 489-498, 1986

Lineage analysis of transplanted individual cells in embryos of drosophila melanogaster iii. commitment and proliferative capabilities of pole cells and midgut progenitors. Wilhelm Roux's Archives of Developmental Biology 195(8): 489-498, 1986

Drosophila nonsense suppressors: functional analysis in Saccharomyces cerevisiae, Drosophila tissue culture cells and Drosophila melanogaster. Genetics 126(3): 625-637, 1990

Mass isolation of pole cells from drosophila melanogaster. Journal of Cell Biology 70(2 PT 2): 196A, 1976

Mass isolation of pole cells from Drosophila melanogaster. Developmental Biology 56(2): 372-381, 1977

Functional Capabilities of a Solitary Kidney Following Resection of 1 Pole and then the other Pole. (Experimental Studies). Urologiia 28: 11-13, 1963

Splicing of the p element in pole cells during embryogenesis of drosophila melanogaster. Zoological Science 8(6): 1068, 1991

Reexamination of the fate of pole cells the precursors to germ cells in drosophila melanogaster. Journal of Cell Biology 83(2 Part 2): 225A, 1979

Splicing of the p element in pole cells during early embryogenesis of drosophila melanogaster. Zoological Science 7(6): 1101, 1990

Fine structure of pole cells and polar granules in Drosophila melanogaster. Jour Exptl Zool 151(3): 201-215, 1962

Lineage analysis of transplanted individual cells in embryos of drosophila melanogaster iv. commitment and proliferative capabilities of mesodermal cells. Wilhelm Roux's Archives of Developmental Biology 196(4): 222-230, 1987

Lineage analysis of transplanted individual cells in embryos of Drosophila melanogaster : IV. Commitment and proliferative capabilities of mesodermal cells. Roux's Archives of Developmental Biology: the Official Organ of the Edbo 196(4): 222-230, 1987

UbcD4, a ubiquitin-conjugating enzyme in Drosophila melanogaster expressed in pole cells. Molecular Genetics and Genomics: Mgg 266(6): 907-913, 2002

Transplantation of pole cells in the drosophila melanogaster female sterile mutant tiny. Journal of Cell Biology 87(2 Part 2): 148A, 1980

Ultrastructure of the ocellar visual system in normal and mutant Drosophila melanogaster. Journal of Neurogenetics 5(2): 127-153, 1989