+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Poly phenol oxidase activity in soybean glycine max hypocotyls at sites inoculated with phytophthora megasperma f sp glycinea



Poly phenol oxidase activity in soybean glycine max hypocotyls at sites inoculated with phytophthora megasperma f sp glycinea



Physiological Plant Pathology 21(2): 227-236



Etiolated 6-day-old soybean hypocotyls were inoculated with P. megasperma f. sp. glycinea by placing drops of zoospore suspensions into the hypocotyl surface. Polyphenoloxidase (PPO) activity at the inoculated sites was determined after various periods of incubation by replacing the inoculum drops with L-.beta.-3,4-dihydroxyphenylalanine (L-dopa) and measuring the formation of dopaquinone spectrophotometrically (.lambda.480 nm). Activity was detected at incompatibly-reacting sites within 2-3 h following inoculation. No activity was detected at compatibly-reacting sites, although these sites developed activity if subsequently reinoculated with an incompatible race. Activity was also demonstrated in hypocotyl tissues injured by abrasion or localized freezing. The development of PPO activity is apparently one of the earliest recognizable events in incompatible interactions, and it may be a consequence of cell damage that is induced by incompatible but not by compatible races.

(PDF emailed within 1 workday: $29.90)

Accession: 006140943

Download citation: RISBibTeXText


Related references

In situ determination of phenol oxidase activity in soybean hypocotyls at sites inoculated with phytophthora megasperma f sp glycinea. Phytopathology 72(2): 263, 1982

Genotype race temperature and cultivar effects on reaction type of unwounded soybean glycine max hypocotyls inoculated with zoo spores of phytophthora megasperma f sp glycinea. Phytopathology 72(7): 801-804, 1982

Resistance susceptibility and accumulation of glyceollins i iii in soybean glycine max organs inoculated with phytophthora megasperma f sp glycinea. Physiological and Molecular Plant Pathology 29(2): 227-238, 1986

Histo pathology of susceptible and resistant soybean glycine max roots inoculated with zoo spores of phytophthora megasperma f sp glycinea. Phytopathology 73(4): 590-595, 1983

Phenylalanine ammonia lyase activity in soybean hypocotyls and leaves following infection with phytophthora megasperma f sp glycinea. Canadian Journal of Botany 66(1): 18-23, 1988

Activated synthesis of poly ribo adenylic acid containing messenger rna in soybean hypocotyls inoculated with phytophthora megasperma var sojae. Physiological Plant Pathology 10(2): 125-138, 1977

Peroxidase synthesis and activity in the interaction of soybean glycine max with phytophthora megasperma f sp glycinea. Plant Physiology (Rockville) 80(4 SUPPL): 131, 1986

Biosynthesis and metabolism of glyceollin i in soybean hypocotyls following wounding or inoculation with phytophthora megasperma f sp glycinea. Physiological & Molecular Plant Pathology 31(3): 387-406, 1987

Identification and accumulation of isoflavonoids and isoflavone glucosides in soybean leaves and hypocotyls in resistance responses to Phytophthora megasperma f.sp. glycinea. Physiological and Molecular Plant Pathology 39(3): 229-244, 1991

Treatment of soybean seed with Ridomil and Captan for control of Phytophthora megsperma f.sp. glycinea, 1981 Soybean (Glycine max Amsoy, Amsoy 71, Corsoy, Corsoy 79, Harcor, Marshall, Pike, Pike II, Pride B216, Steele, A78-125029, A78-227013, A78-227015, A78-227016), pre- and postemergence damping-off and root rot-; Phytophthora megasperma f.sp. glycinea. Fungicide and nematicide tests results American Phytopathological Society7(37): 171-173, 1982

Resistance, susceptibility and accumulation of glyceollins I-III in soybean-organs inoculated with Phytophthora megasperma f.sp. glycinea. Physiological and Molecular Pathology 29(2): 227-237, 1986

Histo pathology of susceptible soybean roots inoculated with zoo spores of phytophthora megasperma f sp glycinea. Phytopathology 72(6): 705, 1982

Reaction of soybean glycine max cultivars to 14 races of phytophthora megasperma f sp glycinea. Plant Disease 67(7): 764-767, 1983

A new allele at the rps 3 locus for resistance to phytophthora megasperma f sp glycinea in soybean glycine max. Phytopathology 75(6): 690-694, 1985

Rps 6 a major gene for resistance to phytophthora megasperma f sp glycinea in soybean glycine max. Phytopathology 72(12): 1564-1567, 1982