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Polyporaceae cellulase and phenol oxidase activities ii. enzymatic activities of coriolus versicolor l. ex fr. quel. 742

Polyporaceae cellulase and phenol oxidase activities ii. enzymatic activities of coriolus versicolor l. ex fr. quel. 742

Boletim da Sociedade Broteriana 59(2): 43-58

A strain (n.degree. 742) of Coriolus versicolor was tested for hydrolytic enzymes degrading insoluble (Avicel, Whatman and Cellulose azure-dyed cotton cellulose) or soluble cellulose (carboxymethylcellulose-CMC). The white-rot fungus, C. versicolor was able to produce clearing zones in Whatman or Avicel cellulose agar and release the dye from the cotton blue cellulose very slowly (after 15 days of culture). The cellulasic activity was also tested with soluble cellulose by the following methods: (1) measurement of discoloured zones (after staining with Congo red) induced by mycelia or extracellular extracts as a function of time, pH and temperature; (2) loss of viscosity of CMC solution; (3) increase in reducing sugars after 4 hours incubation. The phenoloxidasic activity was tested by adding one of nine phenolic compounds to the malt agar and given by colour intensity diameter of the reaction and by the growth of the mycelia in that media.

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