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Post transcriptional regulation of the abundance of messenger rna species encoding alpha tubulin and a 94000 dalton protein in teratocarcinoma derived stem cells vs. differentiated cells


Molecular and Cellular Biology 4(11): 2428-2436
Post transcriptional regulation of the abundance of messenger rna species encoding alpha tubulin and a 94000 dalton protein in teratocarcinoma derived stem cells vs. differentiated cells
Changes in the expression of the genes encoding .alpha.-tubulin and a 94,000-dalton protein (p94) specified by a complementary DNA clone, p4-30, were examined in a differentiated teratocarcinoma-derived parietal endoderm cell line PYs-2, and an undifferentiated teratocarcinoma stem cell line, F9. Relative to other proteins or mRNA species, the synthesis rate of the .alpha.-tubulins and of p94, and the levels of their corresponding cytoplasmic mRNAs, were lower in PYS-2 than in F9 cells. The decrease was greater for the relative abundance of cytoplasmic .alpha.-tubulin mRNA than for p94 mRNA. Induction of differentiation of F9 cells by simultaneous exposure to retinoic acid (RA) and dibutyryl cAMP resulted in reduced relative levels of the cytoplasmic mRNAs for these proteins. The reduction in abundance of the 2 RNA species was not due to a decrease in growth rate since the differentiated cells, PYS-2, RA-treated F9 and RA plus dibutyryl cAMP-treated F9 cells, grew at a rate similar to that of undifferentiated F9 cells. Induction of differentiation of F9 cells by treatment with RA alone did not cause down-regulation of the 2 RNA species. The relative levels of total cellular RNA 3 encoding .alpha.-tubulin and p94 in PYS-2 cells were also lower than those in F9 cells to an extent comparable to the decrease in the cytoplasmic RNAs. Since the apparent relative rates of RNA transcription were similar in both cell types, one concluded that the reduction in the relative levels of the .alpha.-tubulin and p94 RNAs in the cell depends largely on the relative stability of the 2 RNAs and not on the relative rates of transcription. The faster disappearance of the 2 RNA species relative to other cellular RNAs from actinomycin D-treated PYS-2 compared with F9 cells is consistent with this interpretation.


Accession: 006153478



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