EurekaMag.com logo
+ Site Statistics
References:
47,893,527
Abstracts:
28,296,643
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Potassium hydrogen exchange transport in plant membrane vesicles is evidence for potassium transport






Plant Science (Shannon) 41(3): 161-168

Potassium hydrogen exchange transport in plant membrane vesicles is evidence for potassium transport

A tonoplast- and a plasma membrane-enriched vesicle fraction [from zucchini, Cucurbita pepo] both active in H+ transport were prepared with sequential centrifugation on a sucrose step gradient and a glycerol density gradient. At pH 6.5 no differential influence of monovalent cations on H+ transport (.DELTA.pH) was observed whereas at higher pHout a cation-specific decrease of apparent H+ transport activity was observed with the order of apparent activities being Li+ = choline+ > Cs+ > Rb+ > Na+ = K+. The apparent passive proton permeabilities in the presence of these cations increased in the order choline+ = Cs+ < Li+ < Rb+ < Na+ = K+. Allowing electroneutral H+/K+ exchange with nigericin uncoupled the .DELTA.pH but increased the steady state membrane potential. The steady state membrane potential without nigericin at pH 6.5 or at pH 8.0 was highest with K+ as compared to the other alkali cations. This shows that the K+-specific decrease of .DELTA.pH and the increase of the apparent passive proton permeability in the presence of K+ was due to electroneutral K+/H+ exchange transport which might indicate the presence of a K+/H+ antiporter in native plant membranes.


Accession: 006156209



Related references

Scherer, G.G.F.E.; Martiny Baron, G., 1985: K+/H+ exchange transport in plant membrane vesicles is evidence for K+ transport. Demonstrated in tonoplast- and plasma membrane-enriched vesicle fractions from hypocotyl hooks of etiolated Cucurbita pepo seedlings, under alkaline (pH 8.0) conditions.

Skrabanja A.T.P.; D.P.nt J.J.H.H.M.; Bonting S.L., 1984: The hydrogen ion atp transport ratio of the potassium hydrogen ion atpase of pig gastric membrane vesicles. Various values have been reported for the H+/ATP transport ratio of the (K+ + H+)-ATPase of the gastric parietal cell: 4, 2 and 1. This matter was, therefore, reinvestigated with a vesicle preparation isolated from pig gastric mucosa. The vesicles...

Berteloot A., 1986: Membrane potential dependency of glutamic acid transport in rabbit jejunal brush border membrane vesicles potassium and hydrogen effects. We have applied our recently developed approach for quantitative generation and estimation of membrane potential differences (Berteloot, A. (1986) Biochim. Biophys Acta 857, 180-188) to the reevaluation of glutamic acid transport rheogenicity in r...

Nonaka T.; Warden D.H.; Stokes J.B., 1992: Analysis of potassium transport by rabbit ccd conducive pathways and potassium potassium exchange by sodium potassium pump. We studied the cellular pathways of K+ transport by the rabbit cortical collecting duct that was stimulated to absorb Na+ and to secrete K+. The vast majority of K+ secretion (into the lumen) was inhibited by benzamil, a blocker of epithelial Na+...

Weinberg, S.L.; Burckhardt, G.; Wilson, F.A., 1986: Taurocholate transport by rat intestinal basolateral membrane vesicles. Evidence for the presence of an anion exchange transport system. The transport of bile acid was studied in basolateral membrane vesicles isolated from rat small intestine. Taurocholate transport into an osmotically reactive intravesicular space was Na+ independent. The uptake of taurocholate in jejunal and ilea...

Fyles T.M.; Hansen S.P., 1988: Equilibrium and kinetic studies of a chemical model of active transport membrane transport of potassium salts by cryptand carriers driven by potassium ion complexation. A theoretical framework of energy transduction in membranes was used as a point of departure for the design of a simple chemical model of primary active transport (reaction pumping). In the model, cryptand carriers transport potassium salts across...

Hannafin J.; Kinne Saffran E.; Friedman D.; Kinne R., 1983: Ion fluxes and furosemide binding in rectal gland plasma membrane vesicles evidence for the presence of a sodium potassium chloride co transport system. Kidney International 23(1): 257

Kashiwagura T.; Arakawa R.; Takeguchi N., 1982: Hydrogen ion transport and hydrogen ion potassium ion atpase activity in gastric vesicles in osmotically expanded and shrunken states. Cell Structure & Function 7(3): 285-290

Goldman, I.D.; Fyfe, M.J.; Bowen, D.; Loftfield, S.; Schafer, J.A., 1977: The effect of micro tubular inhibitors on transport of alpha amino iso butyric acid inhibition of uphill transport without changes in trans membrane gradients of sodium ion potassium ion or hydrogen ion. Vinca alkaloids partially inhibit the uphill transport of .alpha.-aminoisobutyric acid in [mouse] Ehrlich ascites tumor cells. Maximum inhibition reduced the steady-state .alpha.-aminoisobutyric acid distribution ratio by only 25% leaving a large...

Koenig B.; Ricapito S.; Kinne R., 1983: Chloride transport in the thick ascending limb of henles loop potassium dependence and stoichiometry of the sodium chloride co transport system in plasma membrane vesicles. Cells were isolated from the thick ascending limb of Henle's loop of rabbit kidney outer medulla and a plasma membrane fraction was prepared by differential centrifugation, Na and Rb uptake into the plasma membrane vesicles were determined by...