Preparation and characterization of tritiated derivates of galactosyl ceramide and glucosyl ceramide/
Poulos, A.; Pollard, A.
Journal of Labelled Compounds and Radiopharmaceuticals 14(1): 17-26
ISSN/ISBN: 0362-4803 DOI: 10.1002/jlcr.2580140104
Radiolabeled glucosylceramide and galactosylceramide were prepared by the catalytic tritiation of a mixture of the corresponding unlabeled sphingolipids, and purified by preparative TLC. The localization of the label was determined by degrading the individual monohexosyl ceramides with aqueous methanolic HCl and measuring the radioactivity content of the liberated fragments. Under the particular experimental conditions described, more than 98% of incorporated 3H was found in the ceramide portion of each sphingolipid; the greater part (> 80%) of this radioactivity was detected in the long-chain fatty acid moieties. The method offers a convenient means of preparing labeled substrates suitable for use in metabolic studies. To reduce costs, mixtures, rather than individual sphingolipids, can be tritiated.