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Preparation and cytochemical properties of modified schiff reagents for feulgen staining



Preparation and cytochemical properties of modified schiff reagents for feulgen staining



Life Science Advances 3(2-3): 138-142



This communication reports the use of several organic and inorganic acids along with potassium metabisulphite or sodium thiosulphate for the preparation of modified Schiff reagents for use in Feulgen procedure. It has been observed that all the twelve dye-reagents except salicylic acid-thiosulphate and phospotungstic acid-thiosulphate are highly satisfactory for Feulgen staining of mammalian tissue sections when used under UV rays. All the reagents are also highly suitable when used at elevated temperature. Reaction under UV rays is better than in the controls stained under the usual laboratory conditions. It has been concluded that regeneration of the dye molecules occurs under UV rays as well as under elevated temperature making the dye-reagents similar to an aqueous solution of basic fuchsin which alone produces perfect staining of nuclei in acid hydrolysed tissue sections.

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Accession: 006171616

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