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Preparation and cytochemical properties of per chloric acid and phosphoric acid glycine meta bi sulfite schiff reagents


Indian Journal of Experimental Biology 19(2): 170-172
Preparation and cytochemical properties of per chloric acid and phosphoric acid glycine meta bi sulfite schiff reagents
New methods for the preparation of Schiff reagents employing perchloric or phosphoric acid and the usual potassium metabisulfite were presented. Both the dye-reagents were also fortified with an amino acid, glycine. The staining ability of perchloric acid-Schiff reagent fortified with glycine was slightly less than that of the phosphoric acid-Schiff reagent. The latter dye-reagent when fortified with glycine was easily oxidized on exposure to air at 35.degree. C. The staining reaction with perchloric acid-Schiff reagent started simultaneously with the control, whereas that of phosphoric acid-glycine-Schiff reagent started a bit earlier than its control. The shelf-life of perchloric acid-glycine-Schiff reagent is at least 2 mo. and that of phosphoric acid-glycine-Schiff reagent is at least 6 mo., during which time there was no diminution in Feulgen staining intensity. The implications of these findings are discussed.

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Accession: 006171617



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