EurekaMag.com logo
+ Site Statistics
References:
53,623,987
Abstracts:
29,492,080
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Preparation and properties of creatine kinase from the breast muscle of normal and dystrophic chicken (Gallus domesticus)



Preparation and properties of creatine kinase from the breast muscle of normal and dystrophic chicken (Gallus domesticus)



Biochemical Journal 120(1): 177-185



1. The purification of creatine kinase from normal and genetically dystrophic chicken breast muscle is described. Enzyme recovery was significantly lower from dystrophic muscle. 2. Both enzymes had the same number of reactive and total thiol groups and had similar specific activities and similar amino acid compositions. 3. No significant differences were observed in sedimentation, electrophoretic or kinetic properties. 4. Peptide ;maps' showed no significant differences, and electrophoresis of partial acid hydrolysates of the labelled enzymes suggested that corresponding amino acid sequences around all the thiol groups were very similar. 5. The enzymes showed identical temperature stabilities. 6. No significant differences between the enzymes from normal and dystrophic muscle were observed.

(PDF same-day service: $19.90)

Accession: 006171929

Download citation: RISBibTeXText

PMID: 5494223

DOI: 10.1042/bj1200177



Related references

The amino acid sequence of the peptide containing the thiol group of creatine kinase from normal and dystrophic chicken breast muscle. Comparison of some of the immunological properties of the antibodies developed in rabbits against these enzymes. Biochemical Journal 143(1): 171-179, 1974

Decreased mitochondrial creatine kinase activity in dystrophic chicken breast muscle alters creatine-linked respiratory coupling. Archives of Biochemistry and Biophysics 240(1): 380-391, 1985

Decreased mitochondrial creatine kinase ec 2.7.3.2 activity in dystrophic chicken breast muscle alters creatine linked respiratory coupling. Archives of Biochemistry and Biophysics 240(1): 380-391, 1985

Plasmalogenase activity in normal and dystrophic chicken gallus domesticus erythrocytes. Comparative Biochemistry & Physiology B 79(1): 51-54, 1984

Multiple molecular forms of avian aldolases. I. Crystallization and physical properties of chicken (Gallus domesticus) breast muscle aldolase. Canadian Journal of Biochemistry 47(5): 517-526, 1969

Avian fructose-1,6-diphosphatases. I. Purification and comparison of physical and immunological properties of the liver and breast muscle enzymes from chicken (Gallus domesticus). Biochimica et Biophysica Acta 268(2): 453-467, 1972

Multiple molecular forms of avian aldolases part 1 crystallization and physical properties of chicken gallus domesticus breast muscle aldolase. Canadian Journal of Biochemistry 47(5): 517-526, 1969

Multiple molecular forms of avian aldolases. II. Enzymic properties and amino acid composition of chicken (Gallus domesticus) breast muscle aldolase. Canadian Journal of Biochemistry 47(5): 527-534, 1969

Multiple molecular forms of avian aldolases part 2 enzymic properties and amino acid composition of chicken gallus domesticus breast muscle aldolase. Canadian Journal of Biochemistry 47(5): 527-534, 1969

Genetic characterization of Bhutanese native chickens based on an analysis of Red Junglefowl (Gallus gallus gallus and Gallus gallus spadecieus), domestic Southeast Asian and commercial chicken lines (Gallus gallus domesticus). Genetics and Molecular Biology 35(3): 603-609, 2012

The rates of disappearance of chicken breast muscle amp amino hydrolase and pyruvate kinase from the plasma of normal and genetically dystrophic chickens. Federation Proceedings 39(6): ABSTRACT 2982, 1980