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Preparation of d xylulose from d xylose


, : Preparation of d xylulose from d xylose. Enzyme & Microbial Technology 10(9): 537-542

A simple method is described for the preparation of D-xylulose. It consists of the isomerization of D-xylose with xylose isomerase (EC 5.3.1.5), yielding an equilibrium mixture of D-xylulose and D-xylose. This is followed by the quantitative oxidation of residual D-xylose to D-xylonic acid with immobilized A. calcoaceticus cells. A combination of methanol precipitation and ion exchange is used for the removal of xylonic acid. This procedure offers many advantages over existing methods for the preparation of D-xylulose. The purity of the final product compares favorably to that of a commercial D-xylulose preparation.


Accession: 006173017

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Related references

Chiang, L.C.; Hsiao, H.Y.; Ueng, P.P.; Tsao, G.T., 1981: Enzymatic and Microbial Preparation of d-Xylulose from d-Xylose. A high-d-xylulose mixture (d-xylose-d-xylulose = 33:67) was prepared from the cold ethanol extract of preisomerized d-xylose solution (d-xylose-d-xylulose = 77:23). Fusarium oxysporum f. sp. lini and Aspergillus niger were demonstrated to preferen...

Wang P.Y.; Johnson B.F.; Schneider H., 1980: Fermentation of d xylose by yeasts using glucose isomerase in the medium to convert d xylose to d xylulose. A method to obtain the fermentative conversion by yeasts of D-xylose to ethanol is described. The method depends on a combination of 2 factors; the ability of glucose isomerase to isomerise D-xylose to D-xylulose and the ability of a number of yea...

Evans C.T.; Ratledge C., 1984: Induction of xylulose 5 phosphate phosphoketolase in a variety of yeasts grown on d xylose the key to efficient xylose metabolism. Activity of a pentulose (xylulose 5-phosphate) phosphoketolase was detected in 20 out of 25 yeasts examined. No significant activity was detected in any yeast grown with glucose and the enzyme was induced by up to 70-fold when the yeasts were grow...

Yang, Y.; Hu, C-Wei.; Abu-Omar, M.M., 2012: Synthesis of furfural from xylose, xylan, and biomass using AlCl3·6H2O in biphasic media via xylose isomerization to xylulose. Furfural was prepared in high yields (75 %) from the reaction of xylose in a water-tetrahydrofuran biphasic medium containing AlCl(3)·6H2O and NaCl under microwave heating at 140 °C. The reaction profile revealed the formation of xylulose as an...

Liu, Y.; Rainey, P.B.; Zhang, X-Xian., 2016: Molecular mechanisms of xylose utilization by Pseudomonas fluorescens: overlapping genetic responses to xylose, xylulose, ribose and mannitol. Bacterial degradation of xylose is sequentially mediated by two enzymes - an isomerase (XutA) and a xylulokinase (XutB) - with xylulose as an intermediate. Pseudomonas fluorescens SBW25, though capable of growth on xylose as a sole carbon source...

Olivier, S.P.; Du-Toit, P.J., 1986: Sugarcane bagasse as a possible source of fermentable carbohydrates ii. optimization of the xylose isomerase reaction for isomerization of xylose as well as sugarcane bagasse hydrolysate to xylulose in laboratory scale units. Both the forward and backward reactions of xylose isomerase (Sweetzyme Q) with xylose and glucose as substrates have been studied in terms of kinetics and thermodynamics. The relationship between the two reactions can thus be determined. Much atte...

Hochster, R.M.; Watson, R.W., 1954: Enzymatic isomerization of D-xylose to D-xylulose. Evidence for the specific enzymatic isomerization of D-xylose to D-xylulose in Pseudomonas hydrophila is presented. The enzyme was named xylose isomerase. Several properties of the enzyme are descr. These include heat lability, solubility, substra...

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Tewari Y.B.; Steckler D.K.; Goldberg R.N., 1985: Thermodynamics of the conversion of aqueous xylose to xylulose. The thermodynamics of the conversion of aqueous xylose to xylulose has been investigated using high-pressure liquid chromatography (HPLC) and microcalorimetry. The reaction was carried out in aqueous phosphate buffer over the pH range 6.8-7.4 usin...

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