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Preparation of d xylulose from d xylose


, : Preparation of d xylulose from d xylose. Enzyme & Microbial Technology 10(9): 537-542

A simple method is described for the preparation of D-xylulose. It consists of the isomerization of D-xylose with xylose isomerase (EC 5.3.1.5), yielding an equilibrium mixture of D-xylulose and D-xylose. This is followed by the quantitative oxidation of residual D-xylose to D-xylonic acid with immobilized A. calcoaceticus cells. A combination of methanol precipitation and ion exchange is used for the removal of xylonic acid. This procedure offers many advantages over existing methods for the preparation of D-xylulose. The purity of the final product compares favorably to that of a commercial D-xylulose preparation.

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