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Preparation of desulfated bovine fibrinopeptide b and demonstration of its sulfation in vitro by an enzyme system from neuroblastoma glioma hybrid cells


, : Preparation of desulfated bovine fibrinopeptide b and demonstration of its sulfation in vitro by an enzyme system from neuroblastoma glioma hybrid cells. International Journal of Biochemistry 18(2): 137-142

A method is described for isolating bovine fibrinopeptide B (bFPB) in a highly purified form from crude bovine fibrinogen, using ion-exchange chromatography on DEAE cellulose. Desulphated bFPB (designated DSbFPB) was prepared by treatment of the product with acid. After incubating DSbFPB with [35S]PAPS, in the presence of a particulate preparation from neuroblastoma-glioma hybrid cells, radioactivity was incorporated into a product identified as [35S]bFPB from its position of elution on reverse-phase HPLC. The possible significance of this observation is discussed.

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Barling, P.M.; Palmer, D.J.; Christie, D.L., 1986: Preparation of desulphated bovine fibrinopeptide B and demonstration of its sulphation in vitro by an enzyme system from neuroblastoma-glioma hybrid cells. A method is described for isolating bovine fibrinopeptide B (bFPB) in a highly purified form from crude bovine fibrinogen, using ion-exchange chromatography on DEAE cellulose. Desulphated bFPB (designated DSbFPB) was prepared by treatment of the p...

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