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Preparation of desulfated bovine fibrinopeptide b and demonstration of its sulfation in vitro by an enzyme system from neuroblastoma glioma hybrid cells






International Journal of Biochemistry 18(2): 137-142

Preparation of desulfated bovine fibrinopeptide b and demonstration of its sulfation in vitro by an enzyme system from neuroblastoma glioma hybrid cells

A method is described for isolating bovine fibrinopeptide B (bFPB) in a highly purified form from crude bovine fibrinogen, using ion-exchange chromatography on DEAE cellulose. Desulphated bFPB (designated DSbFPB) was prepared by treatment of the product with acid. After incubating DSbFPB with [35S]PAPS, in the presence of a particulate preparation from neuroblastoma-glioma hybrid cells, radioactivity was incorporated into a product identified as [35S]bFPB from its position of elution on reverse-phase HPLC. The possible significance of this observation is discussed.


Accession: 006173032



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