EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Preparation of different immuno sorbents and their use in isolating antigens and antibodies


Voprosy Meditsinskoi Khimii 22(1): 127-136
Preparation of different immuno sorbents and their use in isolating antigens and antibodies
Immunosorbent preparation methods using amminocellulose, Sephadexes, homo- and heterobifunctional reagents, bis-diazotized aromatic diamine hydrofluorides, glutaric aldehyde, formaldehyde, acrolein, antirat rabbit IgG [immunoglobulin G] and peroxidase-labeled rat IgG, glutaric aldehyde-bound, Escherichia coli, porous granulated glass, preactivated protein and hydrocarbon-containing gels were reviewed. Immunosorbents were used to determine rickettsiosis and ornithosis antigens and Ig and antibody-producing cells, to concentrate smallpox vaccine with the fluorescent antibody method, to isolate antibodies to E. coli and antitetanus antibodies from human blood serum and to detoxicate tetanus and diphtheria toxin. Some methods used normal human and rabbit .gamma.-globulin and bovine and rabbit serum albumin.


Accession: 006173054



Related references

Use of immuno sorbents on the basis of amino anisole cellulose for quantitative determination of antibodies to protein antigens part 1 choosing the immuno sorbent dose and serum volume necessary for complete selective removal of antibodies from it. Lietuvos TSR Mokslu Akademijos Darbai Serija C Biologijos Mokslai (4): 107-114, 1974

A new method for preparation of antibody immuno sorbents to nonprotein antigens. Voprosy Meditsinskoi Khimii 27(6): 844-847, 1981

Express method for quantitative determination of antibodies and antigens using gel immuno sorbents. Laboratornoe Delo 6: 357-360, 1974

Preparation of specific antibodies against mouse platelet antigens by immuno affinity for direct immuno fluorescence of megakaryocytes. Experimental Hematology (Charlottesville) 9(SUPPL 9): 84, 1981

Preparation of insoluble protein derivatives and their use for isolating antibodies, antigens, enzymes and enzyme inhibitors. Comptes Rendus Hebdomadaires des Seances de L'academie des Sciences. Serie D: Sciences Naturelles 268(1): 227-229, 1969

Whole organisms and purified cell walls compared as immuno sorbents for the detection of immuno globulin e antibodies to staphylococcus aureus. Journal of Immunological Methods 66(2): 369-376, 1984

Purification of anti idiotypic antibodies against human cold agglutinins by repeated immuno absorption steps with regenerable immuno sorbents. Immunobiology 160(1): 96, 1982

Use of immuno sorbents on an amino anisole cellulose base for quantitative determination of antibodies to protein antigens part 2 quantitative and qualitative differences between immune sera against proteins of healthy and pathologically altered human heat tissues. Lietuvos TSR Mokslu Akademijos Darbai Serija C Biologijos Mokslai (1): 121-126, 1975

Isolation of kidney tissue antigens from urine by biospecific immuno sorbents. Verhandlungen der Deutschen Gesellschaft fuer Innere Medizin 82(PART II): 1469-1473, 1976

Use of affinity chromatography for isolating antibodies to human brain specific antigens and some data on the characteristics of these antigens. Zhurnal Nevropatologii i Psikhiatrii Imeni S S Korsakova 77(1): 48-51, 1977