EurekaMag.com logo
+ Translate

Preparation of enterochelin from Escherichia coli


, : Preparation of enterochelin from Escherichia coli. Preparative Biochemistry 6(2-3): 123-131

A convenient method has been developed for the preparation of enterochelin, the natural iron carrier produced by Escherichia coli. The method employs a mutant strain which is unable to transport the ferric-enterochelin complex into the cell and which excretes large quantities of enterochelin into the culture medium. The addition of excess iron to the medium allows the enterochelin to accumulate as the ferric-enterochelin complex which is purified by ion-exchange chromatography and then dissociated and the free enterochelin further purified by differential extraction and crystallization. The enterochelin is isolated in good yield and appears to be of high purity as judged by a number of criteria.

(PDF same-day service: $19.90)

Accession: 006173082

PMID: 133343

DOI: 10.1080/00327487608061607

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Greenwood, K.T.; Luke, R.K., 1978: Enzymatic hydrolysis of enterochelin and its iron complex in Escherichia Coli K-12. Properties of enterochelin esterase. Properties of the enzyme which hydrolyses enterochelin (a cyclic trimer of 2,3-dihydroxy-N-benzoyl-L-serine) to 2,3-dihydroxybenzoylserine have been investigated with a view to resolving discrepancies between earlier reports. Enterochelin esterase...

Langman, L.; Young, I.G.; Frost, G.E.; Rosenberg, H.; Gibson, F., 1972: Enterochelin system of iron transport in Escherichia coli: mutations affecting ferric-enterochelin esterase. Three mutant strains of Escherichia coli have been isolated which are lacking ferric-enterochelin esterase activity. This enzyme catalyzes the hydrolysis of the enterochelin moiety of ferric-enterochelin to yield ultimately three molecules of N-2,...

Greenwood, K.T.; Luke, R.J., 1976: Studies on the enzymatic synthesis of enterochelin in Escherichia coli K-12. Four polypeptides involved in the conversion of 2,3-dihydroxybenzoate to enterochelin. Four gene products involved in the enzymatic synthesis of enterochelin from 2,3-dihydroxybenzoate, L-serine and ATP (Luke, R.K.L. and Gibson, F. (1971) J. Bacteriol. 107,557-562; Woodrow, G.C., Young, I.G. and Gibson, F. (1975) J. Bacteriol. 124,...

Porra, R.J.; Langman, L.; Young, I.G.; Gibson, F., 1972: The role of ferric enterochelin esterase in enterochelin-mediated iron transport and ferrochelatase activity in Escherichia coli. Archives of Biochemistry and Biophysics 153(1): 74-78

Greenwood, K.T.; Luke, R.K.J., 1976: Studies on the enzymatic synthesis of enterochelin in escherichia coli k 12 4 poly peptides involved in the conversion of 2 3 di hydroxy benzoate to enterochelin. Four gene products involved in the enzymatic synthesis of enterochelin from 2,3-dihydroxybenzoate, L-serine and ATP were partially purified [from E. coli] using a previously reported fractionation procedure. The products of genes E, F and G were s...

Greenwood, K.T.; Luke, R.K., 1980: Studies on the enzymatic synthesis of enterochelin in Escherichia coli K-12, Salmonella typhimurium and Klebsiella pneumoniae. Physical association of enterochelin synthetase components in vitro. Further evidence is presented in support of the proposal made previously (Greenwood, K.T. and Luke, R.K.J. (1976) Biochim. Biophys. Acta 454, 285-297) that components of the Escherichia coli enterochelin synthetase system physicaloly associate to...

Young, I.G.; Gibson, F., 1979: Isolation of enterochelin from Escherichia coli. Methods in Enzymology 56: 394-398

Cook, G.M.; Loder, C.; Soballe, B., 1998: A factor produced by Escherichia coli K-12 inhibits the growth of E. coli mutants defective in the cytochrome bd quinol oxidase complex: enterochelin rediscovered. Escherichia coli produces an extracellular factor that inhibits the aerobic growth of Cyd- mutants, defective in the synthesis or assembly of the cytochrome bd-type quinol oxidase. This paper shows that such a factor is the iron-chelating sideroph...

Cook, G.M.; Loder, C.; Søballe, B.; Stafford, G.P.; Membrillo-Hernández, J.; Poole, R.K., 1999: A factor produced by Escherichia coli K-12 inhibits the growth of E. coli mutants defective in the cytochrome bd quinol oxidase complex: enterochelin rediscovered. Escherichia coli produces an extracellular factor that inhibits the aerobic growth of Cyd- mutants, defective in the synthesis or assembly of the cytochrome bd-type quinol oxidase. This paper shows that such a factor is the iron-chelating sideroph...

Rogers, H.J.; Synge, C.; Kimber, B.; Bayley, P.M., 1977: Production of enterochelin by escherichia coli strain o 111. The major neutral Fe-transporting compound produced by E. coli 0111/K58/H2 was isolated from Fe-deficient cultures of the organism and compared with the corresponding compound, enterochelin, produced by E. coli K12. The product contained serine an...