+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Preparation of epidermal strips from fossil plants by the peel method

, : Preparation of epidermal strips from fossil plants by the peel method. Annals of Botany (London) 40(170): 1321-1322

During work on silicified rhizome fragments of Phragmites australis (Cav.) Trin. ex Steud (= P. communis Trin.) collected from the Pleistocene of El-Fayum, Egypt, transverse sections of fossil specimens were prepared but it was difficult to distinguish the epidermis in these sections. Because features of the epidermis are more diagnostic than features of other tissues, it was necessary to examine the epidermis of the fossil specimens, as far as possible, in surface view. Since the specimens were somewhat fragile their surface would not stand the usual stages of polishing and etching. These stages were dropped and the technique was started directly by pouring acetone on the surface of the fossil rhizome and gently laying a piece of cellulose acetate film (0.075 mm thick) across the surface of the rhizome, leaving it to dry and then peeling it off as usual in the peel technique. The film obtained by this treatment was comparable to epidermal strips obtained from extant plant specimens. The prepared film made it possible to correct the previous assignation, by Kraeusel (1924) of a similar fossil specimen, from the same locality, to the family Cyperaceae. At places on the rhizome surface where the epidermis was removed the prepared peel, without etching, showed a longitudinal section of the cortical cells. With similarly preserved fossil specimens, for the purpose of preparing cellulose films showing surface view of the epidermis or the outermost layer of cells, it may be worthwhile to prepare the peels directly without polishing and etching.

Accession: 006173090

Submit PDF Full Text: Here

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Related references

El-Saadawi, W.; Badawi, A.; El-Awamri, A., 1976: Preparation of epiderman strips from fossil plants by the peel method. Annals of botany: 40 (170) 1321-1322

Collins Horace R., 1958: The preparation of peel sections of fossil plants found in coal balls. Compass of Sigma Gamma Epsilon, 1915-84 35(3): 207-210

Hudson S.; Trail J.; Simmons D.; Baldwin L.; Myers R.; Tom B.; Mohr J.; Tallman G., 1983: Preparation of enzymatically cleaned leaf epidermal strips of nicotiana glauca. A technique for enzymatically cleaning leaf epidermal strips of N. glauca so that guard cells remain the only intact and viable cells is described. The method relies on differential sensitivities of epidermal, mesophyll and guard cell walls to dig...

Graham Roy, 1933: Preparation of paleobotanical sections by the peel method. Stain Technology 8(2): 65-68

Hara Y.; Fujio Y.; Ueda S., 1985: Ethanol production from raw satsuma mandarin peel without cooking by using enzyme preparation of aspergillus niger cultured in the medium containing peel wastes. Alcoholic fermentation of raw Satsuma mandarin peel was carried out by a nonconventional method. The enzymes used for liquefaction and saccharification were hydrolases of Aspergillus niger cultured in the medium containing Satsuma mandarin peel as...

Fan, J.Z.; Lin, S.X., 1983: A simple method for preparation of long strips from small blood vessels. Sheng Li Ke Xue Jin Zhan 14(4): 372-373

Panichayupakaranant, P.; Itsuriya, A.; Sirikatitham, A., 2010: Preparation method and stability of ellagic acid-rich pomegranate fruit peel extract. A simple one-step purification using liquid-liquid extraction for preparing pomegranate peel extract rich in ellagic acid has been demonstrated. The method involved partitioning of the 10% v/v water in methanol extract of pomegranate peel between...

Levchenko, V.; Guinot, D.R.; Klein, M.; Roelfsema, M.R.G.; Hedrich, R.; Dietrich, P., 2008: Stringent control of cytoplasmic Ca2+ in guard cells of intact plants compared to their counterparts in epidermal strips or guard cell protoplasts. Cytoplasmic calcium elevations, transients, and oscillations are thought to encode information that triggers a variety of physiological responses in plant cells. Yet Ca(2+) signals induced by a single stimulus vary, depending on the physiological...

Spence R.D.; Sharpe P.J.H.; Powell R.D.; Rogers C.A., 1983: Epidermal and guard cell interactions on stomatal aperture in epidermal strips and intact leaves. A study using Vica faba strips and leaf sections revealed the following: a non-linear relationship between aperture and guard cell contact with live epidermal cells; epidermal cell viability on isolated strips had a threshold at about C...

Van-Rensburg, L.; Peacock, J., 1998: Determination of epidermal transpiration in four cultivars of Nicotiana tabacum L. using epidermal strips in a quasi-steady state system. A quasi-steady state method is presented for quantifying epidermal transpiration of epidermal strips where simple relations between transmembrane fluxes and parameters of diffusibility of penetrating compounds hold. Contrary to most permeability s...