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Preparation of erythrocytic ganglioside diagnostic reagent for detecting cholera entero toxin and toxoid in the passive hem agglutination test


, : Preparation of erythrocytic ganglioside diagnostic reagent for detecting cholera entero toxin and toxoid in the passive hem agglutination test. Zhurnal Mikrobiologii Epidemiologii i Immunobiologii (10): 101-104

Cholera enterotoxin and its components (cholerogenoid and subunit B) can be detected in amounts of 0.25, 0.28 and 0.6 .mu.g of protein/ml, respectively, by means of erythrocytes sensitized with a ganglioside-containing complex. The conditions for erythrocyte sensitization were established. Cholerogen titration in the passive hemagglutination test by the erythrocytic ganglioside diagnostic reagent and Craig's skin test correlated. The passive hemagglutination test should be suitable for the detection of other bacterial toxins interacting with gangliosides.

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Dolmatova, L.A.; Efremenko, V.I.; Narbutovich, N.I.; Vladimtseva, I.V., 1981: Production of an erythrocytic ganglioside diagnostic reagent for detecting cholera enterotoxin and toxoid in indirect hemagglutination tests. The studies described in this work have indicated that cholera enterotoxin and its components (cholerogenoid and subunit B) can be detected in amounts of 0.25, 0.28 and 0.6 microgram of protein per 1 ml, respectively, by means of erythrocytes sens...

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