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Preparation of ferritin conjugates and antibodies for the localization and identification of antigens in tissues and cells by electron microscopy


, : Preparation of ferritin conjugates and antibodies for the localization and identification of antigens in tissues and cells by electron microscopy. Scanning Electron Microscopy 4: 17-26

Ferritin, due to its high electron density, serves s a good marker in immunoelectron microscopic studies to localize antigens on and in cells and tissues. A step-by-step methodology for the conjugation of antibodies to ferritin is presented. Special attention has been placed on the techniques for the purification of ferritin and antibodies, conjugation procedures, and the treatment of specimens with conjugates. Methods for obtaining the separation of conjugated antibodies from unconjugated antibodies and for testing the purity and specificity of the labeling reagents are discussed.

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Accession: 006173118

PMID: 6289422

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Coulson, J. M.; Brown, R. A.; Edwards, P. J.; Lewis, F. J., 1990: The effects of tefluthrin on terrestrial non-target organisms. Studies on the effects of tefluthrin applied as a granular or seed treatment on soil microflora, earthworms, beneficial arthropods, birds and mammals are summarized. In the granular formulation, tefluthrin has low toxicity to birds, with even pass...

Ritcey Doug; Hattori Keiko; Wells Gary S.; Riverin Gerald, 1986: Precious metal mineralization in the Big Duck Lake area, District of Thunder Bay, Ontario. Program with Abstracts - Geological Association of Canada(Mineralogical Association of Canada(Canadian Geophysical Union, Joint Annual Meeting 11: 119

Mohri, Y.; Miki, C.; Tanaka, K.; Kawamoto, A.; Ohi, M.; Yokoe, T.; Kusunoki, M., 2013: Clinical correlations and prognostic relevance of tissue angiogenic factors in patients with gastric cancer. Clinical Oncology ) 24(9): 610-616

Gumanenko, E.K.; Kostiuk, G.A.; Boiarintsev, V.V.; Gavrilin, S.V.; Museĭchuk, V.V.; Bikbulatov, V.R., 1998: The successful treatment of a heart wound in severe combined closed trauma. Vestnik Khirurgii Imeni I. I. Grekova 157(4): 103-104

Fiebig, Her, 1969: Das Namur C und Westfal im niederrheinisch-westfalischen Steinkohlengebiet. Compte r. Strat. Geol. Carbon: 79-89

Hesse, G.W.; Shashoua, V.E.; Jacob, J.N., 1985: Inhibitory effect of cholesteryl gamma-aminobutyrate on evoked activity in rat hippocampal slices. Cholesteryl gamma-aminobutyrate (C-G) readily crosses the blood-brain barrier and has properties that suggest that it may be a potential gamma-aminobutyric acid (GABA)-mimetic compound. The effect of this compound on the orthodromically-evoked dis...

ZUCCOLI, G., 1950: The monoreducer: a new device for mortised prosthesis. Rivista Italiana di Stomatologia 5(11): 1513-1521

Dixon, J.M.; Chetty, U.; Forrest, A.P., 1988: Wound infection after breast biopsy. British Journal of Surgery 75(9): 918-919

Anonymous, 1950: Report on the competition for the selection of rice seed 1949. This competition was held by the Vercelli Experiment Station for Rice Cultivation under the auspices of the Italian Ministry of Agriculture and Forests. Among the new highly successful varieties were: Strain 115 obtained from a natural cross betwe...

Basaran, A.; Basaran, M.; Topatan, Bül., 2011: Chorionic villus sampling and the risk of preeclampsia: a systematic review and meta-analysis. Archives of Gynecology and Obstetrics 283(6): 1175-1181