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Preparation of ferritin conjugates and antibodies for the localization and identification of antigens in tissues and cells by electron microscopy


, : Preparation of ferritin conjugates and antibodies for the localization and identification of antigens in tissues and cells by electron microscopy. Scanning Electron Microscopy 4: 17-26

Ferritin, due to its high electron density, serves s a good marker in immunoelectron microscopic studies to localize antigens on and in cells and tissues. A step-by-step methodology for the conjugation of antibodies to ferritin is presented. Special attention has been placed on the techniques for the purification of ferritin and antibodies, conjugation procedures, and the treatment of specimens with conjugates. Methods for obtaining the separation of conjugated antibodies from unconjugated antibodies and for testing the purity and specificity of the labeling reagents are discussed.

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Accession: 006173118

PMID: 6289422

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Related references

Roos, P.; Joseph, M., 1970: Localization of bacterial antigens in electron microscopy with ferritin-labelled antibodies. Annales de L'institut Pasteur de Lille 21: 61-66

Easton, J.M.; Goldberg, B.; Green, H., 1962: Immune cytolysis: electron microscopic localization of cellular antigens with ferritin-antibody conjugates. Immune gamma globulin has been coupled to ferritin by the diisocyanate method of Singer. The final product ("ferroglobulin") had approximately 13 per cent of its gamma globulin coupled to ferritin, and roughly half of its ferritin couple...

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Berthiaume, L.; Micusan, V.; Alain, R.; Trépanier, P., 1981: Rapid identification of viruses by a simple indirect immune electron microscopy technique using ferritin-labelled antibodies. A simple indirect immune-electron microscopy technique using ferritin-labeled antibodies and negative staining to rapidly visualize and identify human viruses is described. The increased electron density given by the ferritin molecules, which also...

Lageman, A.; Dietz, W., 1978: Preparation of hybrid antibodies with mouse immuno globulin g and ferritin specificities for the immune electron microscopic detection of cell membrane antigens. Use of hybrid antibodies with 1 specifity for mouse Ig[immunoglobulin]G and the other 1 for ferritin is particularly suitable for immune electron microscopical detection of cell surface antigens. Preparation of antibodies of such kind was describe...

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Andres, G.; Hsu, K.; Seegal, B., 1973: Immunologic techniques for the identification of antigens or antibodies by electron microscopy. Handbook of Experimental Immunology: 4