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Preparation of glucose from cellulose containing materials using cellulases of different origin


Prikladnaya Biokhimiya i Mikrobiologiya 16(1): 29-39
Preparation of glucose from cellulose containing materials using cellulases of different origin
Hydrolysis of cellulose containing materials and wastes of their industrial processing under the influence of a cellulase, cellokandine G3x, was examined. Among the natural substrates tested - sawdust, cotton, cotton lint, cotton wool, paper and cardboard of different brands (before and after mechanical pretreatment), cotton lint showed the highest reaction capacity during enzymic hydrolysis to yield glucose. The rate and degree of hydrolytic transformation of lint into glucose were investigated under the action of cellulases: cellokandine G3x, celloviridine G3x, cellolignorine P10x, and cellokoningine P10x. The effect of different conditions (pH, temperature, ionic strength of the solution, concentration of the substrate and enzymes as well as the time of cotton lint grinding in a vibromill) on the efficiency of the enzymic hydrolysis of lint was investigated. Hydrolysis of a 20% suspension of ground lint under the influence of cellokoningine P10x for 48 h yielded 10% glucose syrups.


Accession: 006173172



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