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Preparation of glucosyl transferase ec 2.4.1.5 from streptococcus mutans by evolution from water insoluble poly saccharide with a dissociating solvent


, : Preparation of glucosyl transferase ec 2.4.1.5 from streptococcus mutans by evolution from water insoluble poly saccharide with a dissociating solvent. Infection and Immunity 23(2): 446-452

Glucosyltransferase [GTF] (EC 2.4.1.5) was obtained by dissociation from water-insoluble polysaccharide in the presence of 6 M guanidine-hydrochloride. Water-insoluble polysaccharide was synthesized by cell-free culture supernatants from S. mutans strain 6715. Gel filtration of the GTF on a column of 8% agarose in phosphate buffer, followed by filtration on a column of 4% cross-linked agarose in 6 M guanidine-hydrochloride, gave a 23-fold enrichment of the enzyme. The enriched GTF preparation contained 22% carbohydrate and eluted at a position corresponding to a MW of 422,000. Polyacrylamide gel (5%) electrophoresis of this preparation revealed 2 regions which stained for protein, formed water-insoluble polysaccharide in the presence of sucrose, and precipitated with [hamster] antisera directed to crude GTF preparations. The guanidine-eluted enzyme could be primed by 5 .times. 10-5 M dextran T10 (MW 10,000). High-MW glucan, a possible glucan-binding protein and GTF were obtained after the final gel filtration step (4% cross-linked agarose). [The ability to obtain GTF free of other antigens (Ag) in a yield sufficient for immunization experiments may help resolve the role of GTF as a protective Ag in vaccines.].

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Simard, J.-Luc; Cossette, L., J.; Rong, P.-Min; Martinoli, M.-Grazia; Pelletier, G.; Vincent, M., 1992: Isolation of IFAPa-400 cDNAs: Evidence for a transient cytostructural gene activity common to the precursor cells of the myogenic and the neurogenic cell lineages. Differentiation of neural and muscle cells is characterized by a switch in the expression of the type of intermediate filament protein subunit. In these lineages, vimentin is transiently expressed in the initial stages of development and is gradua...

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Nikolajev, G. V., 1976: New synonyms and new species of scarabaeidae from the turkmen ssr ussr coleoptera. Madotrogus (Dasytrogus) kuhitangicus sp. nov. and Hemictenius medvedevi sp. nov. from Turknemia [SSR, USSR] are described. Six new synonyms and 1 new combination are established [Lethrus substriatus Kraatz (= L. turcomanicus Semenov, syn. nov.), L...

Teichmueller Marlies; Teichmuller Rolf, 1968: Cainozoic and Mesozoic coal deposits of Germany. Miocene lignites of Rhine region, Wealden (Cretaceous) coal of Saxony, coal facies, plant assemblages, paleogeographic-climatic-tectonic depositional conditions.

Sankoff, D., 1972: Matching sequences under deletion-insertion constraints. Given two finite sequences, we wish to find the longest common subsequences satisfying certain deletion/insertion constraints. Consider two successive terms in the desired subsequence. The distance between their positions must be the same in the t...

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Feng, H.; Ren, T.; Liu, M.; Yao, K., 2002: Effect of low dose gamma-irradiation and cigarette smoking on growth and reflex behavior in mice. Pregnant mice were exposed to different level of gamma-irradiation(60Co 0, 0.25, 0.5 and 1.0 Gy) and water solutions containing different contents of substance from cigarette smoke(0, 0.5, 1.0, 1.5 and 2.0 cigarette/mouse). Results showed that the...

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